Gtf3a (BC032292) Mouse Tagged Lenti ORF Clone
CAT#: MR201406L4
- LentiORF®
Lenti ORF clone of Gtf3a (mGFP-tagged) - Mouse general transcription factor III A (cDNA clone MGC:40923 IMAGE:5374268)
"BC032292" in other vectors (3)
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CNY 4,750.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | mGFP |
Synonyms | 2010015D03Rik; 2610111I01Rik; 5330403M05Rik |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR201406).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | BC032292 |
ORF Size | 507 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | BC032292, AAH32292 |
RefSeq Size | 996 bp |
RefSeq ORF | 509 bp |
Locus ID | 66596 |
Gene Summary | The product of this gene is a zinc finger protein with nine Cis[2]-His[2] zinc finger domains. It functions as an RNA polymerase III transcription factor to induce transcription of the 5S rRNA genes. The protein binds to a 50 bp internal promoter in the 5S genes called the internal control region (ICR), and nucleates formation of a stable preinitiation complex. This complex recruits the TFIIIC and TFIIIB transcription factors and RNA polymerase III to form the complete transcription complex. The protein is thought to be translated using a non-AUG translation initiation site in mammals based on sequence analysis, protein homology, and the size of the purified protein. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG201406 | Gtf3a (tGFP-tagged) - Mouse general transcription factor III A (cDNA clone MGC:40923 IMAGE:5374268) |
CNY 2,850.00 |
|
MR201406 | Gtf3a (Myc-DDK-tagged) - Mouse general transcription factor III A (cDNA clone MGC:40923 IMAGE:5374268) |
CNY 2,400.00 |
|
MR201406L3 | Lenti ORF clone of Gtf3a (Myc-DDK-tagged) - Mouse general transcription factor III A (cDNA clone MGC:40923 IMAGE:5374268) |
CNY 4,750.00 |