Arf6 (NM_007481) Mouse Tagged ORF Clone
CAT#: MR201520
- TrueORF®
Arf6 (Myc-DDK-tagged) - Mouse ADP-ribosylation factor 6 (Arf6)
ORF Plasmid: tGFP
"NM_007481" in other vectors (4)
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CNY 3,600.00
CNY 4,180.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AI788669; AW496366 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR201520 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAAGGTGCTATCCAAGATCTTCGGGAACAAGGAAATGCGGATCCTCATGCTGGGCCTGGACGCAG CCGGCAAGACAACGATCCTGTACAAGTTGAAGCTGGGCCAATCGGTGACCACCATCCCCACGGTGGGCTT CAACGTGGAGACGGTGACTTACAAAAACGTCAAGTTCAACGTGTGGGATGTGGGCGGCCAGGACAAGATC CGGCCGCTCTGGCGGCATTACTACACCGGGACCCAGGGTCTGATCTTCGTGGTAGACTGCGCCGACCGCG ACCGCATCGACGAGGCCCGCCAGGAGCTGCACCGCATTATCAATGACCGGGAGATGAGGGACGCCATCAT CCTCATCTTCGCCAACAAGCAGGACCTGCCCGATGCCATGAAACCCCATGAGATCCAGGAGAAACTGGGC CTGACCCGGATTCGGGACAGGAACTGGTATGTGCAGCCCTCCTGTGCCACCTCCGGGGACGGACTCTATG AGGGGCTCACATGGTTAACCTCTAACTACAAATCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR201520 protein sequence
Red=Cloning site Green=Tags(s) MGKVLSKIFGNKEMRILMLGLDAAGKTTILYKLKLGQSVTTIPTVGFNVETVTYKNVKFNVWDVGGQDKI RPLWRHYYTGTQGLIFVVDCADRDRIDEARQELHRIINDREMRDAIILIFANKQDLPDAMKPHEIQEKLG LTRIRDRNWYVQPSCATSGDGLYEGLTWLTSNYKS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_007481 |
ORF Size | 528 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007481.3, NP_031507.1 |
RefSeq Size | 3740 bp |
RefSeq ORF | 528 bp |
Locus ID | 11845 |
UniProt ID | P62331 |
MW | 20.1 kDa |
Gene Summary | GTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton remodeling (PubMed:11950392). Required for normal completion of mitotic cytokinesis. Involved in the regulation of dendritic spine development, contributing to the regulation of dendritic branching and filopodia extension. Plays an important role in membrane trafficking, during junctional remodeling and epithelial polarization. Regulates surface levels of adherens junction proteins such as CDH1 (PubMed:29420262, PubMed:20080746). Required for NTRK1 sorting to the recycling pathway from early endosomes (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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ß-Cryptoxanthin reduced lung tumor multiplicity and inhibited lung cancer cells motility by down-regulating nicotinic acetylcholine receptor a7 expression
,Iskandar, AR;Miao, B;Li, X;Hu, KQ;Liu, C;Wang, XD;,
Cancer Prev Res (Phila) 2016
,PubMed ID 27623933
[Arf6]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC208150 | Arf6 (untagged) - Mouse ADP-ribosylation factor 6 (Arf6), (10ug) |
CNY 3,990.00 |
|
MG201520 | Arf6 (tGFP-tagged) - Mouse ADP-ribosylation factor 6 (Arf6) |
CNY 5,200.00 |
|
MR201520L3 | Lenti ORF clone of Arf6 (Myc-DDK-tagged) - Mouse ADP-ribosylation factor 6 (Arf6) |
CNY 5,890.00 |
|
MR201520L4 | Lenti ORF clone of Arf6 (mGFP-tagged) - Mouse ADP-ribosylation factor 6 (Arf6) |
CNY 5,890.00 |