Msrb2 (NM_029619) Mouse Tagged ORF Clone

CAT#: MR201534

  • TrueORF®

Msrb2 (Myc-DDK-tagged) - Mouse methionine sulfoxide reductase B2 (Msrb2)

ORF Plasmid: DDK tGFP



  "NM_029619" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,400.00

CNY 3,705.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 2310050L06Rik; Mrsb; Msrb; Pilb
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR201534 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCGACTGCTGCGAGCGCTGCGGGGTCTGCCTTTGCTGCAGGCGCCTGGACGGCTGGCTCGGGGTT
GCGCGGGATCGGGAAGCAAGGACACAGGGTCTCTTACAAAGTCCAAGCGCTCTCTAAGCGAAGCAGACTG
GCAGAAGAAACTGACCCCGGAACAGTTCTATGTCACCAGAGAGAAAGGAACTGAAGCGCCTTTCAGTGGA
ATGTATTTGAACAACAAGGAGACAGGGATGTACCATTGTGTGTGCTGTGATAGCCCACTCTTCAGTTCTG
AGAAGAAGTACTGTTCTGGCACCGGCTGGCCTTCATTTTCTGAGGCTTACGGCTCAAAAGGCTCAGATGA
AAGTCACACAGGGATCCTGAGACGACTGGACACTTCCTTAGGATGTCCTCGCATGGAGGTGGTCTGCAAG
CAGTGTGAAGCTCACCTTGGCCATGTGTTTCCTGATGGACCCAAGCCCACTGGCCAAAGGTTTTGCATCA
ACAGTGTGGCCCTGAAGTTCAAACCCAGCAAGCCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR201534 protein sequence
Red=Cloning site Green=Tags(s)

MARLLRALRGLPLLQAPGRLARGCAGSGSKDTGSLTKSKRSLSEADWQKKLTPEQFYVTREKGTEAPFSG
MYLNNKETGMYHCVCCDSPLFSSEKKYCSGTGWPSFSEAYGSKGSDESHTGILRRLDTSLGCPRMEVVCK
QCEAHLGHVFPDGPKPTGQRFCINSVALKFKPSKP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_029619
ORF Size 528 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_029619.2, NP_083895.1
RefSeq Size 1204 bp
RefSeq ORF 528 bp
Locus ID 76467
UniProt ID Q78J03
MW 19.2 kDa
Gene Summary Methionine-sulfoxide reductase that specifically reduces methionine (R)-sulfoxide back to methionine. While in many cases, methionine oxidation is the result of random oxidation following oxidative stress, methionine oxidation is also a post-translational modification that takes place on specific residue. Upon oxidative stress, may play a role in the preservation of mitochondrial integrity by decreasing the intracellular reactive oxygen species build-up through its scavenging role, hence contributing to cell survival and protein maintenance.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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