Fam89b (NM_023166) Mouse Tagged ORF Clone
CAT#: MR201546
- TrueORF®
Fam89b (Myc-DDK-tagged) - Mouse family with sequence similarity 89, member B (Fam89b), transcript variant 2
ORF Plasmid: tGFP
"NM_023166" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 1110021A21Rik; MMTV; Mtvr2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR201546 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACGGGCTGCCCGCGACTGAGGCGCCGGGCGGCGCGGGCTGCGCTCTGGCCGGGCTCCCGCCGCTAC CGCGCGGCCTCAGCGGCCTTCTTAACGCTAGTGGGGGCTCGTGGAGGGAGCTGGAGCGTGTCTACAGCCA ACGGAGCCGCATCCACGACGAACTGAGTCGTGCCGCCCGCGCCCCGGATGGTCCTCGCCATGCCGCTGGA TCTGCTAACTCAGGTTCTGCAGCGGGCCCGCGCCGCCCGGTTAACCTCGACTCTGCACTAGCCGCGCTGC GCAAGGAGATGCTGTGGGGCCTGTACGAGTCGATCCAGGACTACAAGCATCTGTGCCAAGACCTGAGCTT GTGCCAGGACCTGTCATCCTCTCTGCACTCGGACAGCTCCTACCCACCTGATGCCGGCCTGTCTGATGAC GAGGAGCCTCCTGATGCCAGCCTGCCCCCAGACCCTCCACCCCTCACCGTACCCCAGACTCACAATGCCC GTGACCAGTGGTTGCAGGATGCTTTCCAAATCAGCCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR201546 protein sequence
Red=Cloning site Green=Tags(s) MNGLPATEAPGGAGCALAGLPPLPRGLSGLLNASGGSWRELERVYSQRSRIHDELSRAARAPDGPRHAAG SANSGSAAGPRRPVNLDSALAALRKEMLWGLYESIQDYKHLCQDLSLCQDLSSSLHSDSSYPPDAGLSDD EEPPDASLPPDPPPLTVPQTHNARDQWLQDAFQISL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_023166 |
ORF Size | 531 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_023166.2 |
RefSeq Size | 1190 bp |
RefSeq ORF | 531 bp |
Locus ID | 17826 |
MW | 18.7 kDa |
Gene Summary | Negatively regulates TGF-beta-induced signaling; in cooperation with SKI prevents the translocation of SMAD2 from the nucleus to the cytoplasm in response to TGF-beta (PubMed:12646588). Acts as an adapter that mediates the specific recognition of LIMK1 by CDC42BPA and CDC42BPB in the lamellipodia. LRAP25-mediated CDC42BPA/CDC42BPB targeting to LIMK1 and the lamellipodium results in LIMK1 activation and the subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation (PubMed:25107909).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC205091 | Fam89b (untagged) - Mouse family with sequence similarity 89, member B (Fam89b), transcript variant 2, (10ug) |
CNY 2,400.00 |
|
MG201546 | Fam89b (tGFP-tagged) - Mouse mammary tumor virus receptor 2 (Mtvr2), transcript variant 2 |
CNY 2,850.00 |
|
MR201546L3 | Lenti ORF clone of Fam89b (Myc-DDK-tagged) - Mouse family with sequence similarity 89, member B (Fam89b), transcript variant 2 |
CNY 4,750.00 |
|
MR201546L4 | Lenti ORF clone of Fam89b (mGFP-tagged) - Mouse family with sequence similarity 89, member B (Fam89b), transcript variant 2 |
CNY 4,750.00 |