Vhl (NM_009507) Mouse Tagged ORF Clone
CAT#: MR201630
- TrueORF®
Vhl (Myc-DDK-tagged) - Mouse von Hippel-Lindau tumor suppressor (Vhl)
ORF Plasmid: tGFP
"NM_009507" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Vhlh |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR201630 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCGGAAGGCAGCCAGTCCAGAGGAGGCGGCGGGGGAGCCCGGTCCTGAGGAGATGGAGGCTGGGC GGCCGCGGCCGGTGCTGCGCTCGGTGAACTCGCGCGAGCCCTCTCAGGTCATCTTCTGCAACCGCAGCCC GCGCGTCGTGCTGCCTTTGTGGCTCAACTTCGACGGCGAGCCTCAGCCCTACCCGATCTTACCACCGGGC ACCGGCCGCCGCATCCACAGCTACCGAGGTCATCTTTGGCTCTTCAGGGATGCGGGGACCCATGATGGAC TTCTGGTTAACCAAACGGAGCTGTTTGTGCCATCCCTCAATGTCGATGGACAGCCTATTTTTGCCAACAT CACATTGCCAGTGTATACCCTGAAAGAGCGGTGCCTTCAGGTTGTGCGGAGCCTGGTCAAGCCTGAGAAC TACAGGAGACTGGACATCGTCAGGTCACTCTATGAGGATTTGGAGGACTACCCAAGTGTGCGGAAGGACA TACAGCGACTGAGCCAAGAGCACCTTGAGAGTCAGCACCTGGAAGAGGAGCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR201630 protein sequence
Red=Cloning site Green=Tags(s) MPRKAASPEEAAGEPGPEEMEAGRPRPVLRSVNSREPSQVIFCNRSPRVVLPLWLNFDGEPQPYPILPPG TGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPEN YRRLDIVRSLYEDLEDYPSVRKDIQRLSQEHLESQHLEEEP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_009507 |
ORF Size | 546 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_009507.4 |
RefSeq Size | 2730 bp |
RefSeq ORF | 546 bp |
Locus ID | 22346 |
UniProt ID | P40338 |
MW | 20.8 kDa |
Gene Summary | Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions. Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases. Ubiquitinates, in an oxygen-responsive manner, ADRB2 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC209640 | Vhl (untagged) - Mouse von Hippel-Lindau tumor suppressor (Vhl), (10ug) |
CNY 2,400.00 |
|
MG201630 | Vhl (tGFP-tagged) - Mouse von Hippel-Lindau syndrome homolog (Vhlh) |
CNY 2,850.00 |
|
MR201630L3 | Lenti ORF clone of Vhl (Myc-DDK-tagged) - Mouse von Hippel-Lindau tumor suppressor (Vhl) |
CNY 4,750.00 |
|
MR201630L4 | Lenti ORF clone of Vhl (mGFP-tagged) - Mouse von Hippel-Lindau tumor suppressor (Vhl) |
CNY 4,750.00 |