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Grem1 (NM_011824) Mouse Tagged ORF Clone
CAT＃: MR201681
- TrueORF®
Grem1 (Myc-DDK-tagged) - Mouse gremlin 1 (Grem1)
ORF Plasmid: tGFP
"NM_011824" in other vectors (6)
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CNY 3,600.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Cktsf1b1; Drm; Grem; ld |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR201681 representing NM_011824.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAATCGCACCGCATACACTGTGGGAGCGTTGCTTCTCCTCCTGGGGACCCTACTGCCAACAGCTGAG GGGAAAAAGAAAGGTTCCCAAGGAGCCATTCCGCCTCCTGACAAGGCTCAGCACAATGACTCTGAGCAG ACCCAGTCCCCACCACAACCTGGCTCCAGGACCCGGGGGCGGGGCCAGGGGCGGGGCACCGCCATGCCT GGAGAGGAGGTGCTTGAGTCCAGCCAAGAGGCCCTGCACGTGACAGAGCGCAAGTATCTGAAGCGAGAT TGGTGCAAAACTCAGCCCCTGAAGCAGACCATCCACGAGGAGGGCTGCAACAGCCGCACTATCATCAAC CGCTTCTGTTATGGCCAGTGCAACTCCTTCTACATCCCCAGGCACATCCGAAAGGAGGAAGGGTCCTTT CAGTCTTGCTCCTTCTGCAAGCCCAAGAAGTTCACCACCATGATGGTCACACTCAACTGTCCTGAGCTA CAGCCACCCACCAAGAAGAAAAGGGTCACACGCGTGAAGCAGTGCCGTTGCATATCCATCGACTTGGAT AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA >Peptide sequence encoded by MR201681
Blue=ORF Red=Cloning site Green=Tag(s) MNRTAYTVGALLLLLGTLLPTAEGKKKGSQGAIPPPDKAQHNDSEQTQSPPQPGSRTRGRGQGRGTAMP GEEVLESSQEALHVTERKYLKRDWCKTQPLKQTIHEEGCNSRTIINRFCYGQCNSFYIPRHIRKEEGSF QSCSFCKPKKFTTMMVTLNCPELQPPTKKKRVTRVKQCRCISIDLD SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011824 |
| ORF Size | 552 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011824.4 |
| RefSeq Size | 1639 bp |
| RefSeq ORF | 555 bp |
| Locus ID | 23892 |
| UniProt ID | O70326 |
| MW | 20.7 kDa |
| Gene Summary | Cytokine that may play an important role during carcinogenesis and metanephric kidney organogenesis, as BMP a antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop (PubMed:12808456, PubMed:15201225). Down-regulates the BMP4 signaling in a dose-dependent manner (PubMed:15133038). Antagonist of BMP2; inhibits BMP2-mediated differentiation of osteoblasts (in vitro) (By similarity). Acts as inhibitor of monocyte chemotaxis (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203822 | Grem1 (untagged) - Mouse gremlin 1 (Grem1), (10ug) |
CNY 2,000.00 |
|
| MG201681 | Grem1 (tGFP-tagged) - Mouse gremlin 1 (Grem1) |
CNY 5,200.00 |
|
| MR201681L1 | Lenti ORF clone of Grem1 (Myc-DDK-tagged) - Mouse gremlin 1 (Grem1) |
CNY 6,000.00 |
|
| MR201681L2 | Lenti ORF clone of Grem1 (mGFP-tagged) - Mouse gremlin 1 (Grem1) |
CNY 4,750.00 |
|
| MR201681L3 | Lenti ORF clone of Grem1 (Myc-DDK-tagged) - Mouse gremlin 1 (Grem1) |
CNY 4,750.00 |
|
| MR201681L4 | Lenti ORF clone of Grem1 (mGFP-tagged) - Mouse gremlin 1 (Grem1) |
CNY 4,750.00 |
