Gpx7 (NM_024198) Mouse Tagged ORF Clone

CAT#: MR201744

  • TrueORF®

Gpx7 (Myc-DDK-tagged) - Mouse glutathione peroxidase 7 (Gpx7)

ORF Plasmid: DDK tGFP



  "NM_024198" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,400.00

CNY 3,705.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 3110050F08Rik; AI327032; GPX6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR201744 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTTGCAGCTGTGGCGACGGCGTGGCTGCTCCTGTGGGCCGCGGCCTGCGCGCAATCCGAGCAGGACT
TCTACGACTTCAAGGCGGTCAACATCCGGGGCAAGCTGGTGTCGCTGGAGAAGTACCGTGGCTCGGTTTC
CCTGGTGGTGAACGTAGCTAGCGAATGTGGCTTCACAGACCAGAACTACCGAGCCTTGCAGCAGCTGCAG
CGGGACCTGGGCCCCCATCATTTTAATGTGCTTGCCTTCCCTTGCAACCAGTTTGGCCAACAGGAACCAG
ACACCAACAGGGAGATTGAGAACTTTGCCCGCCGCACCTACAGTGTCTCTTTTCCCATGTTTAGCAAGAT
CGCAGTCACTGGCACTGGTGCCCACCCTGCCTTCAAGTACCTAACCCAGACTTCTGGGAAGGAGCCCACC
TGGAACTTCTGGAAGTACCTAGTGGACCCAGACGGAAAGGTGGTGGGAGCATGGGACCCCACTGTGCCAG
TGGCGGAGATCAAGCCCCGTATTACAGAGCAGGTGATGAAACTCATCCTTCGGAAACGAGAAGACTTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR201744 protein sequence
Red=Cloning site Green=Tags(s)

MVAAVATAWLLLWAAACAQSEQDFYDFKAVNIRGKLVSLEKYRGSVSLVVNVASECGFTDQNYRALQQLQ
RDLGPHHFNVLAFPCNQFGQQEPDTNREIENFARRTYSVSFPMFSKIAVTGTGAHPAFKYLTQTSGKEPT
WNFWKYLVDPDGKVVGAWDPTVPVAEIKPRITEQVMKLILRKREDL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_024198
ORF Size 561 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_024198.3
RefSeq Size 1214 bp
RefSeq ORF 561 bp
Locus ID 67305
UniProt ID Q99LJ6
MW 21.1 kDa
Gene Summary It protects esophageal epithelia from hydrogen peroxide-induced oxidative stress. It suppresses acidic bile acid-induced reactive oxigen species (ROS) and protects against oxidative DNA damage and double-strand breaks (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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