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Rnf185 (BC014812) Mouse Tagged ORF Clone
CAT＃: MR201843
- TrueORF®
Rnf185 (Myc-DDK-tagged) - Mouse ring finger protein 185 (cDNA clone MGC:19394 IMAGE:3153870)
ORF Plasmid: tGFP
"BC014812" in other vectors (4)
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CNY 2,400.00
CNY 2,945.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC19394 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR201843 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAGTAAAGGGCCTTCGGCCTCTGCATCCACTGAGAATTCCAATGCAGGGGGGCCCAGTGGCAGCA GCAATGGCACTGGTGAGAGTGGAGGGCAGGACAGCACCTTTGAGTGCAACATATGCCTGGACACAGCCAA GGATGCTGTCATCAGCCTGTGTGGCCACCTCTTCTGTTGGCCGTGTTTACATCAGTGGTTGGAGACTAGG CCTAACAGACAAGTGTGTCCAGTCTGCAAAGCTGGCATCAGCCGAGACAAAGTCATCCCCCTCTATGGCA GAGGCAGCACTGGACAGCAGGATCCCAGAGAGAAGACTCCTCCTCGTCCTCAAGGCCAGAGGCCAGAGCC AGAAAATAGAGGGGGATTTCAAGGATTTGGATTTGGAGATGGTGGCTTTCAGATGTCTTTTGGAATTGGA GCATTTCCATTTGGGATATTTGCCACAGCATTTAACATAAATGATGGACGGCCTCCTCCAGCTGTCCCTG GGACACCCCAGTATGTGGATGAGCAGTTCCTGTCACGCCTCTTCCTGTTTGTCGCCCTGGTGATCATGTT CTGGCTCCTGATCGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR201843 protein sequence
Red=Cloning site Green=Tags(s) MASKGPSASASTENSNAGGPSGSSNGTGESGGQDSTFECNICLDTAKDAVISLCGHLFCWPCLHQWLETR PNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREKTPPRPQGQRPEPENRGGFQGFGFGDGGFQMSFGIG AFPFGIFATAFNINDGRPPPAVPGTPQYVDEQFLSRLFLFVALVIMFWLLIA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC014812 |
| ORF Size | 576 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC014812, AAH14812 |
| RefSeq Size | 2736 bp |
| RefSeq ORF | 578 bp |
| Locus ID | 193670 |
| MW | 20.5 kDa |
| Gene Summary | E3 ubiquitin-protein ligase that regulates selective mitochondrial autophagy by mediating 'Lys-63'-linked polyubiquitination of BNIP1. Acts in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway, which targets misfolded proteins that accumulate in the endoplasmic reticulum (ER) for ubiquitination and subsequent proteasome-mediated degradation. Protects cells from ER stress-induced apoptosis. Responsible for the cotranslational ubiquitination and degradation of CFTR in the ERAD pathway. Preferentially associates with the E2 enzymes UBE2J1 and UBE2J2 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC206976 | Rnf185 (untagged) - Mouse ring finger protein 185 (cDNA clone MGC:19394 IMAGE:3153870), (10ug) |
CNY 3,230.00 |
|
| MG201843 | Rnf185 (tGFP-tagged) - Mouse ring finger protein 185 (cDNA clone MGC:19394 IMAGE:3153870) |
CNY 2,850.00 |
|
| MR201843L3 | Lenti ORF clone of Rnf185 (Myc-DDK-tagged) - Mouse ring finger protein 185 (cDNA clone MGC:19394 IMAGE:3153870) |
CNY 4,750.00 |
|
| MR201843L4 | Lenti ORF clone of Rnf185 (mGFP-tagged) - Mouse ring finger protein 185 (cDNA clone MGC:19394 IMAGE:3153870) |
CNY 4,750.00 |
