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Pla2g12a (BC026812) Mouse Tagged ORF Clone
CAT＃: MR201855
- TrueORF®
Pla2g12a (Myc-DDK-tagged) - Mouse phospholipase A2, group XIIA (cDNA clone MGC:25458 IMAGE:4456431)
ORF Plasmid: tGFP
"BC026812" in other vectors (3)
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CNY 2,400.00
CNY 3,030.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | mGXII-1-PLA2, MGC58884, GXII, mGXII-1, Rossy |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR201855 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGACTCCGCGGCCCGCGCCCGCCCGGGGCCCCGCGCTCCTCCTCCTCCTGCTGCTGGCCACTGCGC GCGGGCAGGAACAGGACCAGACCACCGACTGGAGGGCCACCCTCAAGACCATCCGCAACGGCATCCACAA GATAGACACGTACCTCAACGCCGCGCTGGACCTGCTGGGCGGGGAGGACGGGCTCTGCCAGTACAAGTGC AGCGACGGATCGAAGCCTGTTCCACGCTATGGATATAAACCATCTCCACCAAATGGCTGTGGCTCTCCAC TGTTTGGCGTTCATCTGAACATAGGTATCCCTTCCCTGACCAAGTGCTGCAACCAGCACGACAGATGCTA TGAGACCTGCGGGAAAAGCAAGAACGACTGTGACGAGGAGTTCCAGTACTGCCTCTCCAAGATCTGCAGA GACGTGCAGAAGACGCTCGGACTATCTCAGAACGTCCAGGCATGTGAGACAACGGTGGAGCTCCTCTTTG ACAGCGTCATCCATTTAGGCTGCAAGCCATACCTGGACAGCCAGCGGGCTGCATGCTGGTGTCGTTATGA AGAAAAAACAGATCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR201855 protein sequence
Red=Cloning site Green=Tags(s) MVTPRPAPARGPALLLLLLLATARGQEQDQTTDWRATLKTIRNGIHKIDTYLNAALDLLGGEDGLCQYKC SDGSKPVPRYGYKPSPPNGCGSPLFGVHLNIGIPSLTKCCNQHDRCYETCGKSKNDCDEEFQYCLSKICR DVQKTLGLSQNVQACETTVELLFDSVIHLGCKPYLDSQRAACWCRYEEKTDL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC026812 |
| ORF Size | 576 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC026812, AAH26812 |
| RefSeq Size | 1022 bp |
| RefSeq ORF | 578 bp |
| Locus ID | 66350 |
| MW | 21.3 kDa |
| Gene Summary | PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Does not exhibit detectable activity toward sn-2-arachidonoyl- or linoleoyl-phosphatidylcholine or -phosphatidylethanolamine (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG201855 | Pla2g12a (tGFP-tagged) - Mouse phospholipase A2, group XIIA (cDNA clone MGC:25458 IMAGE:4456431) |
CNY 2,850.00 |
|
| MR201855L3 | Lenti ORF clone of Pla2g12a (Myc-DDK-tagged) - Mouse phospholipase A2, group XIIA (cDNA clone MGC:25458 IMAGE:4456431) |
CNY 4,750.00 |
|
| MR201855L4 | Lenti ORF clone of Pla2g12a (mGFP-tagged) - Mouse phospholipase A2, group XIIA (cDNA clone MGC:25458 IMAGE:4456431) |
CNY 4,750.00 |
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