Guca1a (NM_008189) Mouse Tagged ORF Clone
CAT#: MR202081
- TrueORF®
Guca1a (Myc-DDK-tagged) - Mouse guanylate cyclase activator 1a (retina) (Guca1a)
ORF Plasmid: tGFP
"NM_008189" in other vectors (4)
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CNY 2,000.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | GC-A; Gcap1; Guca; Guca1; mGCA; mGCAP1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR202081 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAACATCATGGAGGGCAAGTCCGTGGAAGAGCTGAGCAGCACCGAGTGCCATCAGTGGTATAAGA AATTTATGACGGAGTGCCCATCTGGCCAGCTCACCCTCTACGAATTCCGCCAGTTCTTTGGTCTCAAGAA CCTGAGCCCCTCAGCCAGCCAGTATGTGGAACAGATGTTCGAGACCTTTGACTTCAACAAGGATGGCTAC ATTGACTTCATGGAGTACGTGGCAGCCCTCAGCCTGGTCCTCAAGGGCAAAGTGGAACAGAAGTTGCGCT GGTATTTCAAGCTCTACGACGTCGACGGCAACGGGTGCATAGACAGGGACGAGCTGCTCACCATCATCCG GGCCATCCGAACCATTAATCCCTGGAGCGACTCATCCATGAGTGCCGAGGAATTCACAGATACCGTGTTT GCCAAGATCGACATCAACGGGGATGGGGAACTGTCCCTGGAGGAGTTCATGGAAGGTGTGCAGAAGGACC AGATGCTCCTGGACACGCTGACCCGAAGCCTGGACTTGACCGGCATCGTGCGCAGACTCCAGAACGGCGA ACACGAGGAGGCAGGCACCGGCGACCTGGCAGCGGAGGCTGCGGGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202081 protein sequence
Red=Cloning site Green=Tags(s) MGNIMEGKSVEELSSTECHQWYKKFMTECPSGQLTLYEFRQFFGLKNLSPSASQYVEQMFETFDFNKDGY IDFMEYVAALSLVLKGKVEQKLRWYFKLYDVDGNGCIDRDELLTIIRAIRTINPWSDSSMSAEEFTDTVF AKIDINGDGELSLEEFMEGVQKDQMLLDTLTRSLDLTGIVRRLQNGEHEEAGTGDLAAEAAG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_008189 |
ORF Size | 609 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_008189.3 |
RefSeq Size | 917 bp |
RefSeq ORF | 609 bp |
Locus ID | 14913 |
UniProt ID | P43081 |
MW | 23 kDa |
Gene Summary | This gene encodes a calcium-binding photoreceptor protein. The encoded protein may be involved in modulation of guanylyl cyclase activity, and in turn, the mammalian cone phototransduction cascade. Disruption of this gene results in the retinal degeneration associated with cone dystrophy. [provided by RefSeq, Dec 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC201486 | Guca1a (untagged) - Mouse guanylate cyclase activator 1a (retina) (Guca1a), (10ug) |
CNY 2,400.00 |
|
MG202081 | Guca1a (tGFP-tagged) - Mouse guanylate cyclase activator 1a (retina) (Guca1a) |
CNY 2,190.00 |
|
MR202081L3 | Lenti ORF clone of Guca1a (Myc-DDK-tagged) - Mouse guanylate cyclase activator 1a (retina) (Guca1a) |
CNY 3,900.00 |
|
MR202081L4 | Lenti ORF clone of Guca1a (mGFP-tagged) - Mouse guanylate cyclase activator 1a (retina) (Guca1a) |
CNY 3,900.00 |