Btf3 (NM_145455) Mouse Tagged Lenti ORF Clone
CAT#: MR202110L4
- LentiORF®
Lenti ORF clone of Btf3 (mGFP-tagged) - Mouse basic transcription factor 3 (Btf3), transcript variant 1
"NM_145455" in other vectors (4)
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CNY 3,900.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | mGFP |
Synonyms | 1700054E11Rik |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR202110).
|
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_145455 |
ORF Size | 612 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_145455.3, NP_663430.2 |
RefSeq Size | 1108 bp |
RefSeq ORF | 615 bp |
Locus ID | 218490 |
UniProt ID | Q64152 |
Gene Summary | When associated with NACA, prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. BTF3 is also a general transcription factor that can form a stable complex with RNA polymerase II. Required for the initiation of transcription (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC212687 | Btf3 (untagged) - Mouse basic transcription factor 3 (Btf3), transcript variant 1, (10ug) |
CNY 3,990.00 |
|
MG202110 | Btf3 (tGFP-tagged) - Mouse basic transcription factor 3 (Btf3) |
CNY 2,190.00 |
|
MR202110 | Btf3 (Myc-DDK-tagged) - Mouse basic transcription factor 3 (Btf3), transcript variant 1 |
CNY 2,640.00 |
|
MR202110L3 | Lenti ORF clone of Btf3 (Myc-DDK-tagged) - Mouse basic transcription factor 3 (Btf3), transcript variant 1 |
CNY 3,900.00 |