Dtd1 (NM_025314) Mouse Tagged ORF Clone
CAT#: MR202251
- TrueORF®
Dtd1 (Myc-DDK-tagged) - Mouse D-tyrosyl-tRNA deacylase 1 homolog (S. cerevisiae) (Dtd1), nuclear gene encoding mitochondrial protein
"NM_025314" in other vectors (4)
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CNY 2,400.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 0610006H08Rik; DTD; DUE-B; Hars2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR202251 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGCCGTGGTGCAGCGCGTCACTCGGGCTAGCGTCACAGTTGGAGGAGAGCAGATAAGCGCCATCG GACGGGGCATCTGTGTCTTGCTGGGTATTTCCATGGAAGACTCACAGAAGGAACTGGAACACATGGTTCG AAAGATTTTAAATCTGCGTGTGTTTGAGGACGAGAGTGGGAAGCACTGGTCCAAGAGCGTGATGGATAAG GAGTACGAGGTGCTGTGTGTCAGCCAGTTCACCCTCCAGTGCGTCCTCAAGGGGAACAAGCCCGACTTCC ACCTGGCCATGCCCACAGAGCAGGCAGAGAGCTTCTACAACAGCTTCCTGGAGCAGCTTCGCAAGAGCTA CAGACCAGAGCTTATCAGAGATGGCAAGTTTGGTGCCTACATGCAGGTGCACATTCAGAACGATGGGCCT GTGACTATAGAGCTGGAGTCCCCTGCTCCTGGAGCTGCTAGCTCTGACCCAAAGCAGCTGTCAAAGCTGG AGAAACAGCAGCAGAGAAAAGAAAAGACCAGAGCCAAAGGCCCTTCTGAGTCAAGCAAAGAACGGAATGC ACCCCGCAAAGAAGACCGCAGCGCCAGCAGCGGGGCAGAGGGTGACGTGTCTTCTGAGCGGGAGCCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202251 protein sequence
Red=Cloning site Green=Tags(s) MKAVVQRVTRASVTVGGEQISAIGRGICVLLGISMEDSQKELEHMVRKILNLRVFEDESGKHWSKSVMDK EYEVLCVSQFTLQCVLKGNKPDFHLAMPTEQAESFYNSFLEQLRKSYRPELIRDGKFGAYMQVHIQNDGP VTIELESPAPGAASSDPKQLSKLEKQQQRKEKTRAKGPSESSKERNAPRKEDRSASSGAEGDVSSEREP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_025314 |
ORF Size | 630 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_025314.3, NP_079590.1 |
RefSeq Size | 1283 bp |
RefSeq ORF | 630 bp |
Locus ID | 66044 |
UniProt ID | Q9DD18 |
MW | 23.4 kDa |
Gene Summary | An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA-based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC201829 | Dtd1 (untagged) - Mouse D-tyrosyl-tRNA deacylase 1 homolog (S. cerevisiae) (Dtd1), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 2,400.00 |
|
MC201830 | Dtd1 (untagged) - Mouse D-tyrosyl-tRNA deacylase 1 homolog (S. cerevisiae) (Dtd1), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 2,400.00 |
|
MR202251L3 | Lenti ORF clone of Dtd1 (Myc-DDK-tagged) - Mouse D-tyrosyl-tRNA deacylase 1 homolog (S. cerevisiae) (Dtd1), nuclear gene encoding mitochondrial protein |
CNY 4,750.00 |
|
MR202251L4 | Lenti ORF clone of Dtd1 (mGFP-tagged) - Mouse D-tyrosyl-tRNA deacylase 1 homolog (S. cerevisiae) (Dtd1), nuclear gene encoding mitochondrial protein |
CNY 4,750.00 |