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Wif1 (BC004048) Mouse Tagged ORF Clone
CAT＃: MR202510
- TrueORF®
Wif1 (Myc-DDK-tagged) - Mouse Wnt inhibitory factor 1 (cDNA clone MGC:7686 IMAGE:3497155)
ORF Plasmid: tGFP
"BC004048" in other vectors (3)
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CNY 2,400.00
CNY 2,945.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AW107799; WIF-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR202510 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCAGCCCAGCCTGACCTCAAGGGCACGTCTTTACGCCTTGTTTCTCTCTGGTGCAGCTGAGTGTC CCGGAGGGTGTCGAAATGGAGGCTTTTGTAACGAAAGGCGGGTCTGCGAGTGTCCGGATGGGTTCTACGG GCCTCACTGTGAGAAAGCCCTGTGCATACCCCGATGTATGAACGGTGGTCTGTGTGTCACTCCTGGCTTC TGCATCTGCCCCCCTGGATTCTACGGTGTCAACTGTGACAAAGCAAACTGCTCAACCACCTGCTTTAATG GAGGGACCTGCTTTTACCCGGGAAAATGTATTTGCCCTCCTGGACTCGAGGGAGAGCAGTGTGAACTCAG CAAATGCCCCCAACCCTGCCGAAATGGAGGTAAATGCATTGGTAAAAGCAAGTGTAAGTGCCCGAAAGGT TACCAAGGAGACCTGTGCTCTAAGCCCGTCTGCGAGCCTGGCTGTGGTGCCCACGGAACCTGCCACGAAC CCAACAAGTGCCAGTGTCGAGAGGGCTGGCACGGCAGACACTGCAATAAGAGGTATGGAGCCAGCCTCAT GCATGCCCCGAGGCCAGCAGGCGCCGGGCTGGAGCGACACACGCCTTCACTTAAAAAGGCTGAGGATAGA AGGGATCCACCTGAATCCAATTACATCTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202510 protein sequence
Red=Cloning site Green=Tags(s) MAQPSLTSRARLYALFLSGAAECPGGCRNGGFCNERRVCECPDGFYGPHCEKALCIPRCMNGGLCVTPGF CICPPGFYGVNCDKANCSTTCFNGGTCFYPGKCICPPGLEGEQCELSKCPQPCRNGGKCIGKSKCKCPKG YQGDLCSKPVCEPGCGAHGTCHEPNKCQCREGWHGRHCNKRYGASLMHAPRPAGAGLERHTPSLKKAEDR RDPPESNYIW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC004048 |
| ORF Size | 660 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC004048, AAH04048 |
| RefSeq Size | 1526 bp |
| RefSeq ORF | 662 bp |
| Locus ID | 24117 |
| MW | 23.7 kDa |
| Gene Summary | Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Progenitors oppositely polarize WNT activators and inhibitors to orchestrate tissue development
,null,
eLife
,PubMed ID 32310087
[Wif1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG202510 | Wif1 (tGFP-tagged) - Mouse Wnt inhibitory factor 1 (cDNA clone MGC:7686 IMAGE:3497155) |
CNY 4,000.00 |
|
| MR202510L3 | Lenti ORF clone of Wif1 (Myc-DDK-tagged) - Mouse Wnt inhibitory factor 1 (cDNA clone MGC:7686 IMAGE:3497155) |
CNY 4,750.00 |
|
| MR202510L4 | Lenti ORF clone of Wif1 (mGFP-tagged) - Mouse Wnt inhibitory factor 1 (cDNA clone MGC:7686 IMAGE:3497155) |
CNY 4,750.00 |
