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Sucla2 (BC052774) Mouse Tagged ORF Clone
CAT＃: MR202614
- TrueORF®
Sucla2 (Myc-DDK-tagged) - Mouse succinate-Coenzyme A ligase, ADP-forming, beta subunit (cDNA clone MGC:57012 IMAGE:6465419)
ORF Plasmid: tGFP
"BC052774" in other vectors (4)
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CNY 2,280.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4930547K18Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR202614 representing BC052774
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCAAATTCTATAATCTTTTTCTGAAGCATGATGCAACTATGGTAGAAATAAACCCAATGGTAGAAG ATTCTGACGGAAAGGTGTTGTGCATGGATGCAAAGATCAATTTTGATTCAAATTCAGCCTACCGCCAGAA GAAGATCTTTGATCTTCAGGACTGGAGTCAGGAAGACGAAAGGGACAAAGAAGCAGCTAATGCAGATATT AACTACATCGGCCTCGATGGGAGCATAGGATGTCTAGTAAATGGTGCTGGCTTGGCTATGGCCACAATGG ATATAATAAAACTACACGGAGGGACTCCAGCCAACTTTCTTGATGTTGGTGGAGGCGCCACAGTCCAGCA AGTAACAGAAGCGTTTAAACTCATCACTTCAGATAAAAAGGTGCAGGCTATTCTGGTCAACATTTTTGGA GGAATCATGAGATGTGATGTTATTGCACAAGGAATAGTCATGGCAGTAAAGGATTTAGAAATCAGAATAC CTGTTGTGGTGCGGTTACAAGGTACACGAGTTGATGATGCTAAGGCACTAATAGCAGACAGTGGTCTTAA AATCCTTGCTTGTGATGATTTGGATGAAGCTGCTAAAATGGTTGTAAAGCTCTCTGAAATAGTGACCTTA GCCAAAGAAGCCCATGTGGATGTGAAGTTTCAATTGCCAATA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202614 representing BC052774
Red=Cloning site Green=Tags(s) MIKFYNLFLKHDATMVEINPMVEDSDGKVLCMDAKINFDSNSAYRQKKIFDLQDWSQEDERDKEAANADI NYIGLDGSIGCLVNGAGLAMATMDIIKLHGGTPANFLDVGGGATVQQVTEAFKLITSDKKVQAILVNIFG GIMRCDVIAQGIVMAVKDLEIRIPVVVRLQGTRVDDAKALIADSGLKILACDDLDEAAKMVVKLSEIVTL AKEAHVDVKFQLPI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC052774 |
| ORF Size | 672 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC052774.1 |
| RefSeq Size | 1180 bp |
| RefSeq ORF | 674 bp |
| Locus ID | 20916 |
| MW | 43.2 kDa |
| Gene Summary | ATP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC207074 | Sucla2 (untagged) - Mouse succinate-Coenzyme A ligase, ADP-forming, beta subunit (cDNA clone MGC:57012 IMAGE:6465419), (10ug) |
CNY 3,230.00 |
|
| MG202614 | Sucla2 (tGFP-tagged) - Mouse succinate-Coenzyme A ligase, ADP-forming, beta subunit (cDNA clone MGC:57012 IMAGE:6465419) |
CNY 2,470.00 |
|
| MR202614L3 | Lenti ORF clone of Sucla2 (Myc-DDK-tagged) - Mouse succinate-Coenzyme A ligase, ADP-forming, beta subunit (cDNA clone MGC:57012 IMAGE:6465419) |
CNY 4,180.00 |
|
| MR202614L4 | Lenti ORF clone of Sucla2 (mGFP-tagged) - Mouse succinate-Coenzyme A ligase, ADP-forming, beta subunit (cDNA clone MGC:57012 IMAGE:6465419) |
CNY 4,180.00 |
