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Asah3l (BC059819) Mouse Tagged Lenti ORF Clone
CAT＃: MR202750L4
- LentiORF®
Lenti ORF clone of Asah3l (mGFP-tagged) - Mouse N-acylsphingosine amidohydrolase 3-like (cDNA clone MGC:69583 IMAGE:6839525)
"BC059819" in other vectors (4)
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CNY 4,750.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | mGFP |
| Synonyms | CRG-L1, Acer2, maCER2 |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR202750).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC059819 |
| ORF Size | 687 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC059819, AAH59819 |
| RefSeq Size | 4078 bp |
| RefSeq ORF | 689 bp |
| Locus ID | 230379 |
| Gene Summary | Golgi ceramidase that catalyzes the hydrolysis of ceramides into sphingoid bases like sphingosine and free fatty acids at alkaline pH (PubMed:29401619). Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation (PubMed:29401619). Has a better catalytic efficiency towards unsaturated long-chain ceramides, including C18:1-, C20:1- and C24:1-ceramides (By similarity) (PubMed:29401619). Saturated long-chain ceramides and unsaturated very long-chain ceramides are also good substrates, whereas saturated very long-chain ceramides and short-chain ceramides are poor substrates. Also hydrolyzes dihydroceramides to produce dihydrosphingosine (By similarity). It is the ceramidase that controls the levels of circulating sphingosine-1-phosphate and dihydrosphingosine-1-phosphate in plasma through their production by hematopoietic cells (PubMed:29401619). Regulates cell proliferation, autophagy and apoptosis by the production of sphingosine and sphingosine-1-phosphate. As part of a p53/TP53-dependent pathway, promotes for instance autophagy and apoptosis in response to DNA damage. Through the production of sphingosine, may also regulate the function of the Golgi complex and regulate the glycosylation of proteins (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218013 | Asah3l (untagged) - Mouse N-acylsphingosine amidohydrolase 3-like (cDNA clone MGC:69583 IMAGE:6839525), (10ug) |
CNY 6,940.00 |
|
| MG202750 | Asah3l (tGFP-tagged) - Mouse N-acylsphingosine amidohydrolase 3-like (cDNA clone MGC:69583 IMAGE:6839525) |
CNY 2,850.00 |
|
| MR202750 | Asah3l (Myc-DDK-tagged) - Mouse N-acylsphingosine amidohydrolase 3-like (cDNA clone MGC:69583 IMAGE:6839525) |
CNY 2,400.00 |
|
| MR202750L3 | Lenti ORF clone of Asah3l (Myc-DDK-tagged) - Mouse N-acylsphingosine amidohydrolase 3-like (cDNA clone MGC:69583 IMAGE:6839525) |
CNY 4,750.00 |
