Uchl3 (NM_016723) Mouse Tagged ORF Clone
CAT#: MR202766
- TrueORF®
Uchl3 (Myc-DDK-tagged) - Mouse ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) (Uchl3)
ORF Plasmid: tGFP
"NM_016723" in other vectors (4)
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CNY 2,400.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR202766 representing NM_016723
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGTCAACGCTGGCTGCCGCTGGAGGCCAACCCTGAGGTCACCAACCAGTTTCTCAAGCAGTTAG GCCTGCATCCTAACTGGCAGTTTGTTGATGTGTACGGAATGGAGCCTGAACTTCTTAGCATGGTACCAAG ACCAGTATGCGCAGTGTTACTCCTCTTCCCTATCACAGAAAAGTATGAAGTCTTCAGAACAGAAGAGGAA GAAAAGATAAAATCTCAAGGACAAGATGTGACATCATCAGTATATTTTATGAAACAAACCATCAGCAATG CCTGTGGAACGATTGGACTAATCCATGCCATTGCGAACAACAAAGACAAGATGCACTTCGAATCAGGGTC AACATTGAAAAAGTTCCTGGAGGAGTCTGTATCAATGAGCCCTGAAGAGAGAGCCAAATTCCTGGAGAAC TATGACGCTATTCGAGTTACTCATGAAACCAGTGCACATGAAGGTCAGACTGAGGCACCAAGTATAGATG AAAAAGTAGATCTTCATTTTATTGCGTTAGTACATGTAGATGGGCATCTCTATGAATTAGATGGACGGAA ACCATTTCCAATTAACCATGGGAAAACTAGTGATGAGACGTTGTTAGAGGATGCCATAGAAGTTTGCAAG AAGTTCATGGAACGCGACCCTGATGAGTTAAGATTTAATGCAATTGCTCTCTCAGCGGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202766 representing NM_016723
Red=Cloning site Green=Tags(s) MEGQRWLPLEANPEVTNQFLKQLGLHPNWQFVDVYGMEPELLSMVPRPVCAVLLLFPITEKYEVFRTEEE EKIKSQGQDVTSSVYFMKQTISNACGTIGLIHAIANNKDKMHFESGSTLKKFLEESVSMSPEERAKFLEN YDAIRVTHETSAHEGQTEAPSIDEKVDLHFIALVHVDGHLYELDGRKPFPINHGKTSDETLLEDAIEVCK KFMERDPDELRFNAIALSAA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_016723 |
ORF Size | 690 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_016723.2, NP_057932.2 |
RefSeq Size | 947 bp |
RefSeq ORF | 693 bp |
Locus ID | 50933 |
UniProt ID | Q9JKB1 |
MW | 26.6 kDa |
Gene Summary | Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3", and exhibits a preference towards 'Lys-48'-linked ubiquitin chains. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC209881 | Uchl3 (untagged) - Mouse ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) (Uchl3), (10ug) |
CNY 3,990.00 |
|
MG202766 | Uchl3 (tGFP-tagged) - Mouse ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) (Uchl3) |
CNY 2,850.00 |
|
MR202766L3 | Lenti ORF clone of Uchl3 (Myc-DDK-tagged) - Mouse ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) (Uchl3) |
CNY 4,750.00 |
|
MR202766L4 | Lenti ORF clone of Uchl3 (mGFP-tagged) - Mouse ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) (Uchl3) |
CNY 4,750.00 |