5730409G15Rik (BC051078) Mouse Tagged ORF Clone
CAT#: MR202842
- TrueORF®
5730409G15Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 5730409G15 gene (cDNA clone MGC:59002 IMAGE:6311572)
ORF Plasmid: tGFP
"BC051078" in other vectors (4)
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CNY 2,400.00
CNY 6,365.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 5730409G15Rik; eEF2-KMT; Fam86; Fam86a |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR202842 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCCAAGAGTCCACCCAGTGCCACCGGAGCTATTTGCTGCCTTCAGGAAACTCAGTTACACTCTCTG AGAGCACTGCCATCGTGTCCCATGGCACCACAGGCTTGGTTACATGGGACGCTGCTCTCTACCTGGCAGA ATGGGCCATTGAGAACCCAGCAGCCTTCACTGACAGGACTATCCTGGAGCTAGGCAGTGGAGCTGGCCTT ACAGGCCTGGCAATTTGCAAGGCATGCTGCCCCAGAGCATACATCTTCAGCGACTGTCATGCCCAAGTGC TTGAGCAGCTCCGTGGAAACGTTCTCCTCAACGGCTTCTCCTTAGAGCCGCACACCCCCATTGACGCAGG CAGCTCGAAGGTGACAGTGGCTCAGCTGGACTGGGACGAGGTGACAGCCTCTCAGCTCTCTGCCTTCCAG GCAGATGTTGTCATTGCAGCAGATGTACTGTACTGCTGGGAGATGACACTGTCACTGGTCAGGGTCCTGA AGATGCTCGAGGACTGCCAGAGGAAGAGCGCTCCTGATGTCTATGTAGCCTATACTATCCGCAGCCAGGA CACAGGCAAGCTGTTCATCGAAGAGCTGGACCGTGCTGGAATCTACTGGGAAGAGGTGCCCCCTCACACT GGCAAACTGTTCCCTTACGAAGAGCATTCAGCCATCGTAATCCTGAAGCTGGTGCTGACCAGTCGCCATG GTGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202842 protein sequence
Red=Cloning site Green=Tags(s) MTQESTQCHRSYLLPSGNSVTLSESTAIVSHGTTGLVTWDAALYLAEWAIENPAAFTDRTILELGSGAGL TGLAICKACCPRAYIFSDCHAQVLEQLRGNVLLNGFSLEPHTPIDAGSSKVTVAQLDWDEVTASQLSAFQ ADVVIAADVLYCWEMTLSLVRVLKMLEDCQRKSAPDVYVAYTIRSQDTGKLFIEELDRAGIYWEEVPPHT GKLFPYEEHSAIVILKLVLTSRHGV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | BC051078 |
ORF Size | 705 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC051078, AAH51078 |
RefSeq Size | 1560 bp |
RefSeq ORF | 707 bp |
Locus ID | 70511 |
MW | 25.8 kDa |
Gene Summary | Catalyzes the trimethylation of eukaryotic elongation factor 2 (EEF2) on 'Lys-525'.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC217870 | 5730409G15Rik (untagged) - Mouse RIKEN cDNA 5730409G15 gene (cDNA clone MGC:59002 IMAGE:6311572), (10ug) |
CNY 6,940.00 |
|
MG202842 | 5730409G15Rik (tGFP-tagged) - Mouse RIKEN cDNA 5730409G15 gene (cDNA clone MGC:59002 IMAGE:6311572) |
CNY 2,850.00 |
|
MR202842L3 | Lenti ORF clone of 5730409G15Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 5730409G15 gene (cDNA clone MGC:59002 IMAGE:6311572) |
CNY 4,750.00 |
|
MR202842L4 | Lenti ORF clone of 5730409G15Rik (mGFP-tagged) - Mouse RIKEN cDNA 5730409G15 gene (cDNA clone MGC:59002 IMAGE:6311572) |
CNY 4,750.00 |
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