Kctd6 (NM_027782) Mouse Tagged ORF Clone
CAT#: MR202907
- TrueORF®
Kctd6 (Myc-DDK-tagged) - Mouse potassium channel tetramerisation domain containing 6 (Kctd6)
ORF Plasmid: tGFP
"NM_027782" in other vectors (4)
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CNY 2,400.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 5430433B02Rik; AU044285 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR202907 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATAATGGGGACTGGGGCTATATGATGAGTGACCCAGTCACATTAAATGTAGGTGGACACTTGTACA CGACATCGCTTACCACGTTGACACGCTACCCGGATTCTATGCTTGGAGCTATGTTTGGGGGTGACTTCCC CACAGCCCGAGACCCTCAAGGCAATTACTTCATTGATCGAGACGGACCGCTCTTCCGCTATGTCCTTAAC TTCCTACGGACTTCAGAACTGACACTCCCCCTGGACTTTAAGGAGTTTGATCTGCTTCGGAAAGAGGCTG ATTTCTACCAGATCGAACCCTTGATTCAGTGTCTCAATGACCCCAGGCCTCTGTATCCTATGGATACTTT TGAAGAAGTCGTAGAGCTGTCTAGCACTCGGAAGCTTTCTAAATATTCCAATCCGGTGGCCGTCATCATC ACCCAGTTAACCATCACCACAAAGGTCCACTCCTTACTCGAAGGCATCTCAAACTATTTCACCAAGTGGA ATAAGCACATGATGGACACCAGAGACTGCCAGGTCTCCTTCACCTTTGGACCGTGCGATTACCACCAGGA AGTCTCTCTCCGGGTCCATCTGATGGAGTACATCACGAAGCAAGGTTTCACGATCCGCAATACTCGGGTG CATCACATGAGCGAGCGGGCCAATGAGAACACAGTAGAGCACAACTGGACTTTCTGTAGACTGGCCCGCA AGACGGACGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202907 protein sequence
Red=Cloning site Green=Tags(s) MDNGDWGYMMSDPVTLNVGGHLYTTSLTTLTRYPDSMLGAMFGGDFPTARDPQGNYFIDRDGPLFRYVLN FLRTSELTLPLDFKEFDLLRKEADFYQIEPLIQCLNDPRPLYPMDTFEEVVELSSTRKLSKYSNPVAVII TQLTITTKVHSLLEGISNYFTKWNKHMMDTRDCQVSFTFGPCDYHQEVSLRVHLMEYITKQGFTIRNTRV HHMSERANENTVEHNWTFCRLARKTDD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_027782 |
ORF Size | 714 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_027782.3, NP_082058.1 |
RefSeq Size | 1667 bp |
RefSeq ORF | 714 bp |
Locus ID | 71393 |
UniProt ID | Q8BNL5 |
MW | 27.6 kDa |
Gene Summary | Probable substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex mediating the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes the ubiquitination of HDAC1; the function seems to depend on KCTD11:KCTD6 oligomerization. Can function as antagonist of the Hedgehog pathway by affecting the nuclear transfer of transcription factor GLI1; the function probably occurs via HDAC1 down-regulation, keeping GLI1 acetylated and inactive. Inhibits cell growth and tumorigenicity of medulloblastoma (MDB). Involved in regulating protein levels of ANK1 isoform Mu7 probably implicating CUL3-dependent proteasomal degradation.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC207706 | Kctd6 (untagged) - Mouse potassium channel tetramerisation domain containing 6 (Kctd6), (10ug) |
CNY 3,990.00 |
|
MG202907 | Kctd6 (tGFP-tagged) - Mouse potassium channel tetramerisation domain containing 6 (Kctd6) |
CNY 2,850.00 |
|
MR202907L3 | Lenti ORF clone of Kctd6 (Myc-DDK-tagged) - Mouse potassium channel tetramerisation domain containing 6 (Kctd6) |
CNY 4,750.00 |
|
MR202907L4 | Lenti ORF clone of Kctd6 (mGFP-tagged) - Mouse potassium channel tetramerisation domain containing 6 (Kctd6) |
CNY 4,750.00 |