Pdgfb (NM_011057) Mouse Tagged ORF Clone
CAT#: MR202972
- TrueORF®
Pdgfb (Myc-DDK-tagged) - Mouse platelet derived growth factor, B polypeptide (Pdgfb)
ORF Plasmid: tGFP
"NM_011057" in other vectors (3)
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CNY 3,600.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | PDGF-B; Sis |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR202972 representing NM_011057
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATCGCTGCTGGGCGCTCTTCCTTCCTCTCTGCTGCTACCTGCGTCTGGTCAGCGCCGAGGGGGATC CCATTCCTGAGGAACTGTATGAAATGCTGAGCGACCACTCCATCCGCTCCTTTGATGATCTTCAGCGCCT GCTGCACAGAGACTCCGTAGATGAAGATGGGGCTGAGCTGGACTTGAACATGACCCGAGCACACTCCGGA GTCGAGTTGGAAAGCTCATCTCGAGGGAGGAGGAGCCTAGGGTCCCTGGCAGCAGCAGAGCCTGCTGTAA TCGCCGAGTGCAAGACGCGCACAGAGGTGTTCCAGATCTCTCGGAACCTCATCGATCGCACCAACGCCAA CTTCCTGGTGTGGCCGCCCTGTGTGGAGGTGCAGCGCTGCTCCGGCTGCTGCAATAACCGCAATGTGCAA TGCCGGGCCTCGCAGGTGCAGATGCGGCCGGTCCAGGTGAGAAAGATTGAGATTGTGCGAAAGAAGCCCA TCTTCAAGAAGGCCACAGTGACCTTGGAGGACCACCTCGCCTGCAAGTGTGAGACAGTAGTGACCCCTCG GCCTGTGACTAGAAGTCCTGGGACATCCAGGGAGCAGCGAGCCAAGACGCCTCAAGCTCGGGTGACCATT CGGACGGTGAGAATCCGCCGGCCCCCCAAAGGCAAGCACCGAAAGTTTAAGCACACCCATGACAAGGCGG CCCTGAAGGAGACCCTCGGAGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202972 representing NM_011057
Red=Cloning site Green=Tags(s) MNRCWALFLPLCCYLRLVSAEGDPIPEELYEMLSDHSIRSFDDLQRLLHRDSVDEDGAELDLNMTRAHSG VELESSSRGRRSLGSLAAAEPAVIAECKTRTEVFQISRNLIDRTNANFLVWPPCVEVQRCSGCCNNRNVQ CRASQVQMRPVQVRKIEIVRKKPIFKKATVTLEDHLACKCETVVTPRPVTRSPGTSREQRAKTPQARVTI RTVRIRRPPKGKHRKFKHTHDKAALKETLGA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_011057 |
ORF Size | 723 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_011057.4 |
RefSeq Size | 2956 bp |
RefSeq ORF | 726 bp |
Locus ID | 18591 |
UniProt ID | P31240 |
MW | 27.8 kDa |
Gene Summary | Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA.[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Integration of growth factor gene delivery with collagen-triggered wound repair cascades using collagen-mimetic peptides
,Urello, MA;Kiick, KL;Sullivan, MO;,
Bioeng Transl Med
,PubMed ID 27981245
[PDGFB]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG202972 | Pdgfb (tGFP-tagged) - Mouse platelet derived growth factor, B polypeptide (Pdgfb) |
CNY 5,200.00 |
|
MR202972L3 | Lenti ORF clone of Pdgfb (Myc-DDK-tagged) - Mouse platelet derived growth factor, B polypeptide (Pdgfb) |
CNY 6,000.00 |
|
MR202972L4 | Lenti ORF clone of Pdgfb (mGFP-tagged) - Mouse platelet derived growth factor, B polypeptide (Pdgfb) |
CNY 5,890.00 |