Cenph (NM_021886) Mouse Tagged ORF Clone
CAT#: MR202979
- TrueORF®
Cenph (Myc-DDK-tagged) - Mouse centromere protein H (Cenph)
ORF Plasmid: tGFP
"NM_021886" in other vectors (4)
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CNY 2,400.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 1700021I11Rik; 2410018A12Rik; 2610042E16Rik; 2810046K12Rik; AU044255; CENP-H; ENP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR202979 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAGCAGCCGCGGGAGAGGTCTGAGGCCGGGGCTGAGGCCTGCGAAGAGAAGCGGGGTCTGTCGC AGGCCGCGGAGGAGCGTATCGAGGACCGTATCAGCCTGCTGCTCAGGCTGAGAGCTCAGACAAAACAGCA GCTCTTAGAATATAAGTCCATGATCGACACAAATGAAGAAAAAACTCCAGAACAGATTATGCAAGAAAAA CAGATCGAAGTTAAAATTGAAGAGCTGGAGAATGAAATTGAAGATGTAAAAAGTAATATTGAAATGAAAA GTCTTGCACTGAGCAGGATGAAACTTTCAGTTGCACTTCGGGATAACATGGAGAACATGGGACCTGAGAA TTGTGTGCTCACAGATGACATGAAGCACATATTAAAGCTACAAAAGTTAATAATGAAGTCCCAGGAGGAG TCTTCAGAACTGGAGAAAAAGCTGCTTGATGTCAGAAAGAAGAGACTACAACTAAAGCAAGCTTCAAGAA GTAAGCTTTTAGAAATACAAATCGAGAAGAACAAACAGAAAGAGGATGTCGACAAGATGGAGAATTCTGA AATGATAAAGACCATGAAAAAAAAACTACAGACGGAGATAAAGATCACTACAGTTGTTCAACATACATTC CAGGGCCTTATTTTGGCGAGTAAAACCAACTGGGCAGAGGACCCTGCCCTCAGAGAGACAGTGCTGCAGC TGGAGAAGGACCTCACCACTCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202979 protein sequence
Red=Cloning site Green=Tags(s) MEEQPRERSEAGAEACEEKRGLSQAAEERIEDRISLLLRLRAQTKQQLLEYKSMIDTNEEKTPEQIMQEK QIEVKIEELENEIEDVKSNIEMKSLALSRMKLSVALRDNMENMGPENCVLTDDMKHILKLQKLIMKSQEE SSELEKKLLDVRKKRLQLKQASRSKLLEIQIEKNKQKEDVDKMENSEMIKTMKKKLQTEIKITTVVQHTF QGLILASKTNWAEDPALRETVLQLEKDLTTL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_021886 |
ORF Size | 726 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021886.1, NP_068686.1 |
RefSeq Size | 1239 bp |
RefSeq ORF | 726 bp |
Locus ID | 26886 |
UniProt ID | Q9QYM8 |
MW | 28.1 kDa |
Gene Summary | Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC203160 | Cenph (untagged) - Mouse centromere protein H (Cenph), (10ug) |
CNY 2,400.00 |
|
MG202979 | Cenph (tGFP-tagged) - Mouse centromere protein H (Cenph) |
CNY 2,850.00 |
|
MR202979L3 | Lenti ORF clone of Cenph (Myc-DDK-tagged) - Mouse centromere protein H (Cenph) |
CNY 4,750.00 |
|
MR202979L4 | Lenti ORF clone of Cenph (mGFP-tagged) - Mouse centromere protein H (Cenph) |
CNY 4,750.00 |