United States
Germany
Japan
United Kingdom
China
Lat (NM_010689) Mouse Tagged ORF Clone
CAT＃: MR202998
- TrueORF®
Lat (Myc-DDK-tagged) - Mouse linker for activation of T cells (Lat)
ORF Plasmid: tGFP
"NM_010689" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 2,400.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | p36-38; pp36 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR202998 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGCAGACGCCTTGAGCCCGGTGGGGCTGGGCCTCCTGCTGCTGCCCTTCTTGGTCACGCTCCTGG CTGCCCTGTGCGTGCGCTGCCGTGAGTTGCCAGTCTCCTATGACAGCACTTCCACAGAGAGTTTGTACCC AAGAAGCATCCTCATCAAGCCACCTCAAATAACCGTCCCCCGAACACCTGCTGTTTCCTACCCTCTAGTC ACTTCCTTCCCACCCCTGAGGCAGCCAGACCTGCTCCCCATCCCGAGATCCCCACAGCCCCTTGGGGGTT CCCATCGGATGCCATCTTCCCAGCAGAATTCAGATGATGCCAACAGTGTGGCAAGCTACGAGAACCAGGA GCCAGCCTGTAAGAATGTGGATGCAGATGAGGATGAAGACGACTATCCCAACGGCTACCTAGTGGTGCTG CCTGACAGTAGTCCTGCTGCCGTCCCTGTTGTCTCCTCTGCTCCTGTGCCTAGCAACCCTGACCTTGGAG ACAGTGCCTTCTCTGTGGAGTCGTGTGAAGATTACGTGAATGTTCCTGAGAGTGAGGAGAGCGCAGAGGC GTCTCTGGATGGGAGCCGGGAGTATGTGAATGTGTCCCCAGAGCAGCAGCCAGTGACCAGGGCTGAGCTG GCCTCTGTGAACTCCCAGGAGGTGGAAGACGAAGGAGAAGAGGAAGGGGTGGATGGAGAGGAAGCTCCCG ACTATGAGAATCTACAGGAGCTTAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202998 protein sequence
Red=Cloning site Green=Tags(s) MEADALSPVGLGLLLLPFLVTLLAALCVRCRELPVSYDSTSTESLYPRSILIKPPQITVPRTPAVSYPLV TSFPPLRQPDLLPIPRSPQPLGGSHRMPSSQQNSDDANSVASYENQEPACKNVDADEDEDDYPNGYLVVL PDSSPAAVPVVSSAPVPSNPDLGDSAFSVESCEDYVNVPESEESAEASLDGSREYVNVSPEQQPVTRAEL ASVNSQEVEDEGEEEGVDGEEAPDYENLQELN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010689 |
| ORF Size | 729 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_010689.2, NP_034819.1 |
| RefSeq Size | 1260 bp |
| RefSeq ORF | 729 bp |
| Locus ID | 16797 |
| UniProt ID | O54957 |
| MW | 26 kDa |
| Gene Summary | Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200797 | Lat (untagged) - Mouse linker for activation of T cells (Lat), (10ug) |
CNY 2,400.00 |
|
| MG202998 | Lat (tGFP-tagged) - Mouse linker for activation of T cells (Lat) |
CNY 2,850.00 |
|
| MR202998L3 | Lenti ORF clone of Lat (Myc-DDK-tagged) - Mouse linker for activation of T cells (Lat) |
CNY 4,750.00 |
|
| MR202998L4 | Lenti ORF clone of Lat (mGFP-tagged) - Mouse linker for activation of T cells (Lat) |
CNY 4,750.00 |
