Nmnat3 (NM_144533) Mouse Tagged ORF Clone
CAT#: MR203070
- TrueORF®
Nmnat3 (Myc-DDK-tagged) - Mouse nicotinamide nucleotide adenylyltransferase 3 (Nmnat3), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_144533" in other vectors (4)
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CNY 2,400.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 4933408N02Rik; PNAT3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR203070 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAACCGAATCCCTGTGGTGCTTCTGGCCTGTGGTTCCTTCAACCCCATCACGAATATGCACCTGC GCTTGTTTGAGGTGGCCAGAGACCACCTACACCAAACAGGAAGGTACCAGGTGATTGAGGGCATCATCTC ACCCGTCAATGACAGCTATGGGAAGAAAGACCTGGTGGCTTCCCATCACCGAGTGGCCATGGCCCGGCTG GCCCTGCAGACATCTGACTGGATTCGGGTGGACCCCTGGGAGAGTGAGCAGGCGCAGTGGATGGAAACGG TGAAGGTGCTGAGGCACCATCACAGGGAGCTGCTCAGATCCTCAGCCCAGATGGATGGCCCAGACCCCAG CAAGACACCATCAGCCTCTGCAGCACTGCCAGAGTTGAAACTCCTCTGCGGAGCTGATGTCCTCAAGACC TTCCAGACCCCCAACCTCTGGAAAGACACGCACATCCAGGAAATAGTGGAGAAGTTCGGCTTGGTGTGCG TGAGCAGGAGCGGTCATGACCCGGAAAGGTACATCTCGGACTCGCCCATCCTCCAGCAGTTTCAGCACAA CATTCACCTGGCCAGGGAACCCGTTCTGAACGAGATCAGTGCCACATACGTCAGGAAAGCCTTGGGACAA GGGCAGAGCGTGAAGTACCTCCTCCCTGAGGCCGTCATCACCTACATCAGGGACCAGGGCCTCTACATCA ATGACGGTTCCTGGAAAGGGAAAGGAAAGACTGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203070 protein sequence
Red=Cloning site Green=Tags(s) MKNRIPVVLLACGSFNPITNMHLRLFEVARDHLHQTGRYQVIEGIISPVNDSYGKKDLVASHHRVAMARL ALQTSDWIRVDPWESEQAQWMETVKVLRHHHRELLRSSAQMDGPDPSKTPSASAALPELKLLCGADVLKT FQTPNLWKDTHIQEIVEKFGLVCVSRSGHDPERYISDSPILQQFQHNIHLAREPVLNEISATYVRKALGQ GQSVKYLLPEAVITYIRDQGLYINDGSWKGKGKTG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_144533 |
ORF Size | 738 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_144533.3 |
RefSeq Size | 2160 bp |
RefSeq ORF | 738 bp |
Locus ID | 74080 |
UniProt ID | Q99JR6 |
MW | 27.7 kDa |
Gene Summary | Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency. Can use triazofurin monophosphate (TrMP) as substrate. Can also use GTP and ITP as nucleotide donors. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity, can use NAD(+), NADH, NaAD, nicotinic acid adenine dinucleotide phosphate (NHD), nicotinamide guanine dinucleotide (NGD) as substrates. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NaADP(+). Protects against axonal degeneration following injury.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200575 | Nmnat3 (untagged) - Mouse nicotinamide nucleotide adenylyltransferase 3 (Nmnat3), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 2,400.00 |
|
MG203070 | Nmnat3 (tGFP-tagged) - Mouse nicotinamide nucleotide adenylyltransferase 3 (Nmnat3) |
CNY 2,850.00 |
|
MR203070L3 | Lenti ORF clone of Nmnat3 (Myc-DDK-tagged) - Mouse nicotinamide nucleotide adenylyltransferase 3 (Nmnat3), nuclear gene encoding mitochondrial protein |
CNY 4,750.00 |
|
MR203070L4 | Lenti ORF clone of Nmnat3 (mGFP-tagged) - Mouse nicotinamide nucleotide adenylyltransferase 3 (Nmnat3), nuclear gene encoding mitochondrial protein |
CNY 4,750.00 |