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Mbnl1 (BC096600) Mouse Tagged ORF Clone
CAT＃: MR203170
- TrueORF®
Mbnl1 (Myc-DDK-tagged) - Mouse muscleblind-like 1 (Drosophila) (cDNA clone MGC:106037 IMAGE:4976244)
ORF Plasmid: tGFP
"BC096600" in other vectors (4)
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CNY 2,400.00
CNY 6,365.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Mbnl, mKIAA0428 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR203170 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCATGCTGGCCCAGCAAATGCAGTTAGCCAATGCCATGATGCCCGGTGCCCCGTTGCAGCCCGTGC CAATGTTTTCAGTTGCACCAAGCTTAGCCACCAGTGCATCAGCAGCCTTTAACCCTTACCTGGGGCCTGT TTCCCCAAGCCTGGTTCCAGCAGAGATCTTGCCGACTGCACCAATGTTGGTCACGGGGAATCCTGGAGTT CCAGTGCCAGCAGCTGCCGCAGCTGCTGCACAGAAGTTAATGCGGACAGACAGACTGGAGGTGTGTCGAG AGTACCAGCGTGGCAATTGCAACAGAGGAGAAAATGACTGTCGGTTTGCTCATCCTGCTGACAGCACAAT GATTGATACCAATGACAACACAGTCACTGTCTGCATGGATTACATCAAGGGGAGATGCTCTCGGGAAAAG TGCAAATACTTCCATCCTCCCGCACACCTGCAAGCCAAGATCAAGGCTGCCCAATACCAGGTCAACCAGG CTGCAGCAGCACAGGCTGCAGCTACTGCAGCTGCCATGGGAATTCCTCAAGCTGTACTTCCCCCATTGCC AAAGAGGCCTGCTCTTGAAAAAACCAACGGTGCCACCGCAGTCTTTAACACTGGTATTTTCCAATACCAA CAGGCTCTAGCCAACATGCAGTTACAGCAGCATACAGCATTTCTCCCACCAGGCTCAATATTGTGCATGA CACCCGCTACAAGTGTTGATACCCATAATATCTGCCGAACATCTGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203170 protein sequence
Red=Cloning site Green=Tags(s) MAMLAQQMQLANAMMPGAPLQPVPMFSVAPSLATSASAAFNPYLGPVSPSLVPAEILPTAPMLVTGNPGV PVPAAAAAAAQKLMRTDRLEVCREYQRGNCNRGENDCRFAHPADSTMIDTNDNTVTVCMDYIKGRCSREK CKYFHPPAHLQAKIKAAQYQVNQAAAAQAAATAAAMGIPQAVLPPLPKRPALEKTNGATAVFNTGIFQYQ QALANMQLQQHTAFLPPGSILCMTPATSVDTHNICRTSD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC096600 |
| ORF Size | 747 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC096600, AAH96600 |
| RefSeq Size | 4055 bp |
| RefSeq ORF | 749 bp |
| Locus ID | 56758 |
| MW | 26.4 kDa |
| Gene Summary | Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5'-YGCU(U/G)Y-3'consensus sequence. Binds to the IR RNA. Binds to CUG triplet repeat expansion in myotonic dystrophy muscle cells by sequestering the target RNAs (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218336 | Mbnl1 (untagged) - Mouse muscleblind-like 1 (Drosophila) (cDNA clone MGC:106037 IMAGE:4976244), (10ug) |
CNY 2,640.00 |
|
| MG203170 | Mbnl1 (tGFP-tagged) - Mouse muscleblind-like 1 (Drosophila) (cDNA clone MGC:106037 IMAGE:4976244) |
CNY 2,850.00 |
|
| MR203170L3 | Lenti ORF clone of Mbnl1 (Myc-DDK-tagged) - Mouse muscleblind-like 1 (Drosophila) (cDNA clone MGC:106037 IMAGE:4976244) |
CNY 4,750.00 |
|
| MR203170L4 | Lenti ORF clone of Mbnl1 (mGFP-tagged) - Mouse muscleblind-like 1 (Drosophila) (cDNA clone MGC:106037 IMAGE:4976244) |
CNY 4,750.00 |
