Clic5 (NM_172621) Mouse Tagged ORF Clone
CAT#: MR203209
- TrueORF®
Clic5 (Myc-DDK-tagged) - Mouse chloride intracellular channel 5 (Clic5), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_172621" in other vectors (4)
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CNY 2,400.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 5730531E12Rik; B330005L24; Gm322; jbg |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR203209 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGACTCAGCGACAACTAATGGGGACGACAGGGACCCCGAGATCGAGCTCTTCGTGAAGGCTGGGA TCGACGGGGAAAGCATTGGCAACTGTCCCTTCTCTCAGCGTCTCTTCATGATCCTCTGGCTGAAAGGAGT CGTGTTCAATGTCACCACCGTGGATCTGAAAAGAAAGCCAGCCGATCTACACAACCTGGCTCCTGGCACG CACCCGCCCTTCCTGACCTTCAATGGGGATGTGAAGACAGATGTCAACAAGATTGAAGAGTTCCTGGAGG AGACCCTAACCCCCGAGAAGTACCCCAAACTGGCTGCAAAACACCGGGAATCTAACACCGCGGGCATCGA CATCTTCTCCAAGTTCTCAGCCTACATCAAAAACACCAAACAACAGAACAATGCTGCCCTTGAGAGAGGC TTGACAAAGGCGCTGAGGAAGCTGGATGACTACCTAAACAGCCCTCTGCCAGAGGAGATTGACACCAACA CCCACGGGGACGAGAAGGGGTCCCAGCGCAAGTTCCTGGATGGGGATGAGCTGACCCTGGCCGACTGCAA TCTGCTGCCCAAGCTGCATGTGGTCAAGATTGTGGCTAAGAAGTACCGAAACTATGACATCCCAGCTGAG ATGACAGGCTTGTGGCGATACCTCAAGAATGCCTATGCACGGGACGAGTTCACCAACACCTGTGCAGCTG ACAGTGAGATCGAGTTGGCCTACGCAGATGTCGCCAGGCGCCTCAGCCGATCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203209 protein sequence
Red=Cloning site Green=Tags(s) MTDSATTNGDDRDPEIELFVKAGIDGESIGNCPFSQRLFMILWLKGVVFNVTTVDLKRKPADLHNLAPGT HPPFLTFNGDVKTDVNKIEEFLEETLTPEKYPKLAAKHRESNTAGIDIFSKFSAYIKNTKQQNNAALERG LTKALRKLDDYLNSPLPEEIDTNTHGDEKGSQRKFLDGDELTLADCNLLPKLHVVKIVAKKYRNYDIPAE MTGLWRYLKNAYARDEFTNTCAADSEIELAYADVARRLSRS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_172621 |
ORF Size | 756 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_172621.2, NP_766209.1 |
RefSeq Size | 5870 bp |
RefSeq ORF | 756 bp |
Locus ID | 224796 |
UniProt ID | Q8BXK9 |
MW | 28.3 kDa |
Gene Summary | Required for normal hearing (By similarity). It is necessary for the formation of stereocilia in the inner ear and normal development of the organ of Corti. Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. May play a role in the regulation of transepithelial ion absorption and secretion. Is required for the development and/or maintenance of the proper glomerular endothelial cell and podocyte architecture (PubMed:17021174). Plays a role in formation of the lens suture in the eye, which is important for normal optical properties of the lens (PubMed:29425878).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC207893 | Clic5 (untagged) - Mouse chloride intracellular channel 5 (Clic5), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 3,990.00 |
|
MG203209 | Clic5 (tGFP-tagged) - Mouse chloride intracellular channel 5 (Clic5) |
CNY 2,850.00 |
|
MR203209L3 | Lenti ORF clone of Clic5 (Myc-DDK-tagged) - Mouse chloride intracellular channel 5 (Clic5), nuclear gene encoding mitochondrial protein |
CNY 4,750.00 |
|
MR203209L4 | Lenti ORF clone of Clic5 (mGFP-tagged) - Mouse chloride intracellular channel 5 (Clic5), nuclear gene encoding mitochondrial protein |
CNY 4,750.00 |