Psmd8 (BC004075) Mouse Tagged ORF Clone

CAT#: MR203305

  • TrueORF®

Psmd8 (Myc-DDK-tagged) - Mouse proteasome (prosome, macropain) 26S subunit, non-ATPase, 8 (cDNA clone MGC:8242 IMAGE:3591796), complete

ORF Plasmid: DDK tGFP



  "BC004075" in other vectors (4)

Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 2,400.00

CNY 2,570.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 6720456J22Rik; AA407360; AL033291; AL033322; AL033323; C76433
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR203305 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTACGAGCAACTCAAGGACGAGTGGAACCGGAAGAGCCCGAACCTTAGTAAATGTGGAGAAGAACTGG
GCCGCCTGAAGTTGGTTCTGCTTGAGCTCAATTTCCTGCCAACCACGGGGACCAAACTGACTAAACAGCA
ACTCATTCTGGCCCGTGACATTCTGGAGATCGGGGCCCAGTGGAGCATCCTGTGCAAAGACATTCCCTCC
TTCGAGCGCTACATGGCCCAACTCAAATGCTACTACTTTGATTACAAAGAACAGCTTCCTGAGTCAGCCT
ATATGCACCAGCTCCTGGGCCTCAATCTCCTCTTCCTGCTATCTCAGAACCGAGTGGCTGAGTTCCACAC
AGAATTAGAACGATTACCTGCCAAGGACATCCAGACCAATGTCTACATCAAACACCCTGTTTCCCTCGAG
CAATACCTCATGGAGGGTAGCTACAACAAGGTATTCCTGGCTAAGGGGAACATCCCTGCCGAAAGCTACA
CCTTCTTCATTGACATCCTGCTGGATACCATCAGGGATGAAATCGCAGGATGCATTGAGAAGGCCTATGA
GAAAATCCTTTTTGCTGAGGCTACCCGGATCCTCTTCTTCAGCACACCCAAAAAGATGACAGACTATGCC
AAGAAGCGAGGTTGGGTGCTGGGCCCTAACAACTACTACAGCTTTGCCAGCCAGCAGCAGAAGCCAGAAG
ACAGCACCATCCCCTCTACTGAACTGGCCAAACAAGTCATCGAGTATGCCCGGCAGCTGGAGATGATTGT
C


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR203305 protein sequence
Red=Cloning site Green=Tags(s)

MYEQLKDEWNRKSPNLSKCGEELGRLKLVLLELNFLPTTGTKLTKQQLILARDILEIGAQWSILCKDIPS
FERYMAQLKCYYFDYKEQLPESAYMHQLLGLNLLFLLSQNRVAEFHTELERLPAKDIQTNVYIKHPVSLE
QYLMEGSYNKVFLAKGNIPAESYTFFIDILLDTIRDEIAGCIEKAYEKILFAEATRILFFSTPKKMTDYA
KKRGWVLGPNNYYSFASQQQKPEDSTIPSTELAKQVIEYARQLEMIV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC004075
ORF Size 771 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC004075.1
RefSeq Size 1284 bp
RefSeq ORF 773 bp
Locus ID 57296
MW 29.9 kDa
Gene Summary Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...