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Tmem55a (NM_028264) Mouse Tagged ORF Clone
CAT＃: MR203329
- TrueORF®
Tmem55a (Myc-DDK-tagged) - Mouse transmembrane protein 55A (Tmem55a)
ORF Plasmid: tGFP
"NM_028264" in other vectors (3)
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CNY 2,400.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2610319K07Rik; AI315591; AV001360; Pip4p2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR203329 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCTGACGGGGTGGACGAACGTTCGCCTCTGCTGTCAGCATCCCATTCGGGAAATGTCACTCCCA CAGCCCCGCCGTACTTGCAGGAAAGCAGCCCCAGAGCTGAACTCCCACCTCCGTATACAGCCATCGCCAG TCCAGGAACAAGTGGTATTCCCGTGATCAACTGTCGTGTGTGCCAATCTCTAATCAACCTGGATGGTAAA CTTCACCAGCATGTGGTTAAGTGCACAGTTTGCAATGAAGCTACGCCAATCAAAACCCCCCCAACAGGGA AGAAATATGTTAGATGCCCTTGTAATTGTCTACTCATTTGTAAGGATACATCTCGGCGAATAGGATGTCC GAGACCCAACTGTCGACGCATAATTAACCTTGGCCCCGTAATGCTCATTTCTGAAGAGCAACCAGCCCAA CCTGCATTGCCGATCCAGCCAGAAGGCACAAGGGTAGTGTGCGGGCACTGCGGGAACACATTCCTGTGGA TGGAACTGAGGTTCAACACTCTGGCAAAATGCCCACACTGCAAAAAAATCTCCTCGGTAGGTAGCGCCCT TCCCCGGAGACGCTGCTGTGCGTATGTCACCATCGGAATGATATGTATTTTCATTGCAGTTGGATTAACT GTTGGCACACAAGATTTTTCAAGGCGATTTCATGCAACCTATGTGTCTTGGGCAATTGCTTATCTGCTAG GTTTGATTTGCCTTATCCGAGCTTGTTACTGGGGCGCAATCAGAGTCAGTTATCCAGAGCACGGCTTTGC T ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203329 protein sequence
Red=Cloning site Green=Tags(s) MAADGVDERSPLLSASHSGNVTPTAPPYLQESSPRAELPPPYTAIASPGTSGIPVINCRVCQSLINLDGK LHQHVVKCTVCNEATPIKTPPTGKKYVRCPCNCLLICKDTSRRIGCPRPNCRRIINLGPVMLISEEQPAQ PALPIQPEGTRVVCGHCGNTFLWMELRFNTLAKCPHCKKISSVGSALPRRRCCAYVTIGMICIFIAVGLT VGTQDFSRRFHATYVSWAIAYLLGLICLIRACYWGAIRVSYPEHGFA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_028264 |
| ORF Size | 774 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_028264.4 |
| RefSeq Size | 2280 bp |
| RefSeq ORF | 774 bp |
| Locus ID | 72519 |
| UniProt ID | Q9CZX7 |
| MW | 28 kDa |
| Gene Summary | Catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate (PtdIns-4,5-P2) to phosphatidylinositol-4-phosphate (PtdIns-4-P) (By similarity). Does not hydrolyze phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-bisphosphate, inositol 3,5-bisphosphate, inositol 3,4-bisphosphate, phosphatidylinositol 5-monophosphate, phosphatidylinositol 4-monophosphate and phosphatidylinositol 3-monophosphate (By similarity). Negatively regulates the phagocytosis of large particles by reducing phagosomal phosphatidylinositol 4,5-bisphosphate accumulation during cup formation (PubMed:29378918).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG203329 | Tmem55a (tGFP-tagged) - Mouse transmembrane protein 55A (Tmem55a) |
CNY 4,000.00 |
|
| MR203329L3 | Lenti ORF clone of Tmem55a (Myc-DDK-tagged) - Mouse transmembrane protein 55A (Tmem55a) |
CNY 4,750.00 |
|
| MR203329L4 | Lenti ORF clone of Tmem55a (mGFP-tagged) - Mouse transmembrane protein 55A (Tmem55a) |
CNY 4,750.00 |
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