Comt (NM_007744) Mouse Tagged ORF Clone
CAT#: MR203511
- TrueORF®
Comt (Myc-DDK-tagged) - Mouse catechol-O-methyltransferase (Comt), transcript variant 2
ORF Plasmid: tGFP
"NM_007744" in other vectors (6)
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CNY 2,400.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Comt1; D16Wsu103e; D330014B15Rik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR203511 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGTTGGCTGCTGTCTCATTGGGTCTCCTGTTGCTGGCCTTCCTCCTGCTCCTGCGACACCTAGGCT GGGGCTTGGTGGCTATTGGTTGGTTTGAGTTCGTGCAGCAGCCGGTCCACAACCTGCTCATGGGTGGCAC AAAGGAGCAGCGCATCCTGCGCCATGTGCAGCAACACGCAAAGCCTGGAGACCCCCAGAGCGTCCTGGAG GCCATTGATACCTACTGCTCAGAGAAGGAGTGGGCCATGAACGTGGGTGACGCAAAAGGCCAAATCATGG ATGCAGTGATTCGGGAGTACAGGCCCTCGCTGGTGCTGGAGCTAGGAGCTTATTGTGGCTACTCAGCCGT GCGAATGGCCCGCCTGCTGCCACCTGGAGCCAGGCTTCTCACCATGGAGATTAACCCTGACTACGCTGCC ATCACCCAGCAAATGCTGGACTTCGCAGGCCTACAGGACAAAGTTTCCATCCTCATCGGGGCATCCCAGG ACCTTATCCCCCAGCTGAAGAAGAAGTACGATGTGGACACATTAGACATGGTCTTTCTTGACCACTGGAA AGACCGCTACCTTCCAGACACACTTCTCCTGGAGGAATGTGGCCTGCTGCGCAAGGGGACGGTGCTCCTA GCTGACAATGTCATTGTCCCGGGAACCCCTGACTTCCTGGCGTATGTGAGGGGGAGCAGCAGCTTCGAGT GCACACACTACAGCTCATACCTGGAGTACATGAAAGTGGTGGACGGCTTGGAGAAGGCAGTCTACCAGGG TCCAGGCAGCAGCCCCGTGAAGTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203511 protein sequence
Red=Cloning site Green=Tags(s) MLLAAVSLGLLLLAFLLLLRHLGWGLVAIGWFEFVQQPVHNLLMGGTKEQRILRHVQQHAKPGDPQSVLE AIDTYCSEKEWAMNVGDAKGQIMDAVIREYRPSLVLELGAYCGYSAVRMARLLPPGARLLTMEINPDYAA ITQQMLDFAGLQDKVSILIGASQDLIPQLKKKYDVDTLDMVFLDHWKDRYLPDTLLLEECGLLRKGTVLL ADNVIVPGTPDFLAYVRGSSSFECTHYSSYLEYMKVVDGLEKAVYQGPGSSPVKS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_007744 |
ORF Size | 798 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007744.3 |
RefSeq Size | 2128 bp |
RefSeq ORF | 798 bp |
Locus ID | 12846 |
UniProt ID | O88587 |
MW | 29.5 kDa |
Gene Summary | Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200816 | Comt (untagged) - Mouse catechol-O-methyltransferase (Comt), transcript variant 2, (10ug) |
CNY 2,400.00 |
|
MG203511 | Comt (tGFP-tagged) - Mouse catechol-O-methyltransferase (Comt) |
CNY 2,850.00 |
|
MR203511L1 | Lenti ORF clone of Comt (Myc-DDK-tagged) - Mouse catechol-O-methyltransferase (Comt), transcript variant 2 |
CNY 4,750.00 |
|
MR203511L2 | Lenti ORF clone of Comt (mGFP-tagged) - Mouse catechol-O-methyltransferase (Comt), transcript variant 2 |
CNY 4,800.00 |
|
MR203511L3 | Lenti ORF clone of Comt (Myc-DDK-tagged) - Mouse catechol-O-methyltransferase (Comt), transcript variant 2 |
CNY 4,750.00 |
|
MR203511L4 | Lenti ORF clone of Comt (mGFP-tagged) - Mouse catechol-O-methyltransferase (Comt), transcript variant 2 |
CNY 4,750.00 |