United States
Germany
Japan
United Kingdom
China
Gpm6a (BC023461) Mouse Tagged ORF Clone
CAT＃: MR203545
- TrueORF®
Gpm6a (Myc-DDK-tagged) - Mouse glycoprotein m6a (cDNA clone MGC:32434 IMAGE:5041793)
ORF Plasmid: tGFP
"BC023461" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 2,400.00
CNY 6,365.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC38999, M6A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR203545 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGTGCTTCGAGTGCTGCATTAAATGCCTGGGAGGTATTCCCTATGCTTCTCTGATTGCAACCATCC TGCTGTATGCAGGCGTTGCCCTGTTCTGTGGCTGTGGCCATGAAGCCCTTTCTGGAACAGTCAACATTCT GCAGACCTACTTTGAGTTGGCAAGGACTGCTGGAGACACACTGGATGTTTTCACTATGATTGACATCTTT AAGTATGTGATCTATGGCATTGCGGCTGCTTTCTTTGTCTATGGCATTTTACTGATGGTAGAAGGTTTCT TCACAACTGGGGCTATCAAAGATCTCTATGGAGACTTCAAAATCACCACCTGTGGCAGATGTGTGAGCGC TTGGTTTATCATGCTGACATACCTCTTCATGTTGGCCTGGCTGGGAGTCACAGCTTTCACCTCACTGCCC GTGTACATGTATTTCAATGTGTGGACCATCTGCCGGAACACCACTCTAGTGGAGGGAGCAAATCTCTGCT TGGATCTGCGTCAGTTTGGGATTGTGACAATTGGAGAGGAAAAGAAAATTTGTACTGCCTCTGAGAACTT CCTGAGGATGTGTGAATCTACTGAGCTGAATATGACCTTCCACTTGTTCATTGTGGCACTTGCTGGAGCT GGAGCAGCAGTTATTGCTATGGTCCACTACCTGATGGTTCTGTCTGCCAACTGGGCCTATGTGAAAGATG CCTGCCGCATGCAGAAGTACGAAGACATCAAGTCAAAGGAAGAGCAGGAGCTGCACGACATCCATTCTAC TCGCTCTAAAGAGCGGCTCAATGCGTACACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203545 protein sequence
Red=Cloning site Green=Tags(s) MGCFECCIKCLGGIPYASLIATILLYAGVALFCGCGHEALSGTVNILQTYFELARTAGDTLDVFTMIDIF KYVIYGIAAAFFVYGILLMVEGFFTTGAIKDLYGDFKITTCGRCVSAWFIMLTYLFMLAWLGVTAFTSLP VYMYFNVWTICRNTTLVEGANLCLDLRQFGIVTIGEEKKICTASENFLRMCESTELNMTFHLFIVALAGA GAAVIAMVHYLMVLSANWAYVKDACRMQKYEDIKSKEEQELHDIHSTRSKERLNAYT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC023461 |
| ORF Size | 801 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC023461, AAH23461 |
| RefSeq Size | 2780 bp |
| RefSeq ORF | 803 bp |
| Locus ID | 234267 |
| MW | 29.8 kDa |
| Gene Summary | Involved in neuronal differentiation, including differentiation and migration of neuronal stem cells. Plays a role in neuronal plasticity and is involved in neurite and filopodia outgrowth, filopodia motility and probably synapse formation. Gpm6a-induced filopodia formation involves mitogen-activated protein kinase (MAPK) and Src signaling pathways. Conflictingly, PubMed:22162747 reports that induced cellular protrusions are simple membrane-wrapped tubules without actin or tubulin-based cytoskeletons and with Gpm6a gliding along membrane edges indicative for a function in actin-independent membrane deformation. May be involved in neuronal NGF-dependent Ca(2+) influx. May be involved in regulation of endocytosis and intracellular trafficking of G-protein-coupled receptors (GPCRs); enhances internalization and recycling of mu-type opioid receptor.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
A mammalian nervous-system-specific plasma membrane proteasome complex that modulates neuronal function
,Ramachandran, KV;Margolis, SS;,
Nat. Struct. Mol. Biol
,PubMed ID 28287632
[GPM6A]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG203545 | Gpm6a (tGFP-tagged) - Mouse glycoprotein m6a (cDNA clone MGC:32434 IMAGE:5041793) |
CNY 2,850.00 |
|
| MR203545L3 | Lenti ORF clone of Gpm6a (Myc-DDK-tagged) - Mouse glycoprotein m6a (cDNA clone MGC:32434 IMAGE:5041793) |
CNY 4,750.00 |
|
| MR203545L4 | Lenti ORF clone of Gpm6a (mGFP-tagged) - Mouse glycoprotein m6a (cDNA clone MGC:32434 IMAGE:5041793) |
CNY 4,750.00 |
