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Ctdspl (NM_133710) Mouse Tagged ORF Clone
CAT＃: MR203704
- TrueORF®
Ctdspl (Myc-DDK-tagged) - Mouse CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase-like (Ctdspl)
"NM_133710" in other vectors (3)
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CNY 1,416.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2810418J22Rik; AI426263; HYA22; SCP3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR203704 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGGTCCGGCCATCATCACCCAGGTGACCAACCCCAAGGAGGATGAGGCCCGGTCGCCCGTCGCGG GCGAAAAAGCTTCCCAGCGCAACATCAGCCTAAAGAAGCAAAGGGGTCGGAGCATCCTTAGCTCTTTCTT CTGCTGCTTCCGAGACTACAATGTGGAAGCCCCTCCGGCCAACAGCCCCAGTGTGCTTCCGCCACTGGTG GAGGAGAACGGTGGGCTTCAGAAGGGTGACCAGAGGCAGGTCATTCCCGTACCAAGCCCACCAGCTAAAT ACCTCCTTCCAGAGGTGACGGTGCTCGACTATGGAAAGAAATGTGTGGTCATTGATCTAGACGAAACCCT GGTGCACAGTTCGTTTAAGCCCATTAGTAATGCTGACTTCATTGTTCCTGTTGAAATCGACGGAACCATA CACCAGGTGTATGTGCTGAAGCGACCTCACGTGGACGAGTTCCTCCAGAGGATGGGGCAGCTCTTTGAGT GTGTGCTCTTTACTGCCAGCTTGGCCAAGTATGCAGACCCTGTTGCCGACCTCCTGGACCGCTGGGGTGT GTTCCGGGCCCGACTCTTCAGAGAATCCTGCGTGTTCCACCGTGGGAACTATGTGAAGGACTTGAGTCGC CTGGGACGGGAGCTGAGCAAAGTGATCATCGTGGACAATTCTCCTGCCTCCTACATCTTCCATCCTGAGA ATGCAGTGCCCGTGCAGTCCTGGTTTGACGACATGACAGACACGGAGCTGTTGGACCTCATCCCGTTCTT CGAGGGCCTGAGCAGGGAAGACGATGTCTACAGCATGCTGCACAGACTCTGTAGCAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203704 protein sequence
Red=Cloning site Green=Tags(s) MDGPAIITQVTNPKEDEARSPVAGEKASQRNISLKKQRGRSILSSFFCCFRDYNVEAPPANSPSVLPPLV EENGGLQKGDQRQVIPVPSPPAKYLLPEVTVLDYGKKCVVIDLDETLVHSSFKPISNADFIVPVEIDGTI HQVYVLKRPHVDEFLQRMGQLFECVLFTASLAKYADPVADLLDRWGVFRARLFRESCVFHRGNYVKDLSR LGRELSKVIIVDNSPASYIFHPENAVPVQSWFDDMTDTELLDLIPFFEGLSREDDVYSMLHRLCSR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133710 |
| ORF Size | 831 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133710.3, NP_598471.3 |
| RefSeq Size | 4627 bp |
| RefSeq ORF | 831 bp |
| Locus ID | 69274 |
| UniProt ID | P58465 |
| MW | 31.2 kDa |
| Gene Summary | Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC207681 | Ctdspl (untagged) - Mouse CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase-like (Ctdspl), (10ug) |
CNY 3,990.00 |
|
| MR203704L3 | Lenti ORF clone of Ctdspl (Myc-DDK-tagged) - Mouse CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase-like (Ctdspl) |
CNY 4,750.00 |
|
| MR203704L4 | Lenti ORF clone of Ctdspl (mGFP-tagged) - Mouse CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase-like (Ctdspl) |
CNY 4,750.00 |
