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Hes1 (BC018375) Mouse Tagged ORF Clone
CAT＃: MR203819
- TrueORF®
Hes1 (Myc-DDK-tagged) - Mouse hairy and enhancer of split 1 (Drosophila) (cDNA clone MGC:25356 IMAGE:4505427)
ORF Plasmid: tGFP
"BC018375" in other vectors (3)
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CNY 2,400.00
CNY 6,365.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | bHLHb39; Hry |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR203819 representing BC018375
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGCTGATATAATGGAGAAAAATTCCTCCTCCCCGGTGGCTGCTACCCCAGCCAGTGTCAACACGA CACCGGACAAACCAAAGACGGCCTCTGAGCACAGAAAGTCATCAAAGCCTATCATGGAGAAGAGGCGAAG GGCAAGAATAAATGAAAGTCTAAGCCAACTGAAAACACTGATTTTGGATGCACTTAAGAAAGATAGCTCC CGGCATTCCAAGCTAGAGAAGGCAGACATTCTGGAAATGACTGTGAAGCACCTCCGGAACCTGCAGCGGG CGCAGATGACCGCCGCGCTCAGCACAGACCCGAGCGTGTTGGGGAAATACCGCGCCGGCTTCAGCGAGTG CATGAACGAGGTGACCCGCTTCCTGTCCACGTGTGAGGGCGTTAACACCGAGGTGCGCACTCGGCTGCTG GGCCACCTGGCCAACTGCATGACCCAGATCAACGCCATGACCTACCCCGGGCAGGCGCACCCCGCCTTGC AGGCGCCGCCGCCGCCGCCCCCGTCAGGACCTGCCGGTCCCCAGCACGCGCCATTCGCGCCGCCGCCGCC GCCGCTTGTGCCCATCCCCGGGGGCGCGGCGCCCCCTCCCGGCAGCGCACCCTGCAAGTTGGGCAGCCAG GCTGGAGAGGCTGCCAAGGTTTTTGGCGGCTTCCAAGTGGTGCCGGCTCCTGACGGCCAATTTGCCTTTC TCATCCCCAACGGGGCCTTCGCTCACAGCGGCCCGGTCATCCCGGTCTACACCAGCAACAGTGGGACCTC GGTGGGTCCTAACGCAGTGTCACCTTCCAGTGGCTCCTCGCTCACTTCGGACTCCATGTGGAGACCGTGG CGGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203819 representing BC018375
Red=Cloning site Green=Tags(s) MPADIMEKNSSSPVAATPASVNTTPDKPKTASEHRKSSKPIMEKRRRARINESLSQLKTLILDALKKDSS RHSKLEKADILEMTVKHLRNLQRAQMTAALSTDPSVLGKYRAGFSECMNEVTRFLSTCEGVNTEVRTRLL GHLANCMTQINAMTYPGQAHPALQAPPPPPPSGPAGPQHAPFAPPPPPLVPIPGGAAPPPGSAPCKLGSQ AGEAAKVFGGFQVVPAPDGQFAFLIPNGAFAHSGPVIPVYTSNSGTSVGPNAVSPSSGSSLTSDSMWRPW RN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC018375 |
| ORF Size | 846 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC018375.1 |
| RefSeq Size | 1487 bp |
| RefSeq ORF | 848 bp |
| Locus ID | 15205 |
| MW | 29.7 kDa |
| Gene Summary | Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1 (By similarity). Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity. May play a role in a functional FA core complex response to DNA cross-link damage, being required for the stability and nuclear localization of FA core complex proteins, as well as for FANCD2 monoubiquitination in response to DNA damage (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG203819 | Hes1 (tGFP-tagged) - Mouse hairy and enhancer of split 1 (Drosophila) (cDNA clone MGC:25356 IMAGE:4505427) |
CNY 2,850.00 |
|
| MR203819L3 | Lenti ORF clone of Hes1 (Myc-DDK-tagged) - Mouse hairy and enhancer of split 1 (Drosophila) (cDNA clone MGC:25356 IMAGE:4505427) |
CNY 4,750.00 |
|
| MR203819L4 | Lenti ORF clone of Hes1 (mGFP-tagged) - Mouse hairy and enhancer of split 1 (Drosophila) (cDNA clone MGC:25356 IMAGE:4505427) |
CNY 4,750.00 |
