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Ccnd2 (NM_009829) Mouse Tagged ORF Clone
CAT＃: MR203949
- TrueORF®
Ccnd2 (Myc-DDK-tagged) - Mouse cyclin D2 (Ccnd2)
ORF Plasmid: tGFP
"NM_009829" in other vectors (3)
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CNY 2,400.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2600016F06Rik; AI256817; BF642806; C86853; cD2; Vin-1; Vin1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR203949 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGCTGTGCTGCGAGGTGGACCCGGTCCGCAGGGCCGTGCCGGACCGCAACCTGCTGGAAGACC GCGTTCTGCAGAACCTGTTGACCATCGAGGAGCGCTACCTCCCGCAGTGTTCCTATTTCAAGTGCGTGCA GAAGGACATCCAACCGTACATGCGCAGGATGGTGGCCACCTGGATGCTAGAGGTCTGTGAGGAACAAAAG TGTGAAGAAGAGGTCTTTCCTCTGGCCATGAATTACCTGGACCGTTTCTTGGCTGGAGTCCCGACTCCTA AGACCCATCTTCAGCTCCTGGGTGCAGTGTGCATGTTCCTAGCTTCCAAGCTGAAAGAGACCATCCCGCT GACTGCGGAAAAGCTGTGCATTTACACCGACAACTCTGTGAAGCCCCAGGAGCTGCTGGAGTGGGAACTG GTAGTGTTGGGTAAGCTGAAGTGGAACCTGGCCGCAGTCACCCCTCACGACTTCATTGAGCACATCCTTC GCAAGCTGCCCCAGCAAAAGGAGAAGCTGTCCCTGATCCGCAAGCATGCGCAGACCTTCATCGCTCTGTG CGCTACCGACTTCAAGTTTGCCATGTACCCGCCGTCGATGATTGCAACTGGAAGCGTGGGAGCAGCCATC TGTGGGCTTCAGCAGGATGATGAAGTGAACACACTCACGTGTGATGCCCTGACAGAGCTGCTGGCCAAGA TCACCCACACTGATGTGGATTGTCTCAAAGCCTGCCAGGAGCAAATCGAAGCTCTGCTGCTGAACAGCCT GCAGCAGTTCCGTCAAGAGCAGCATAACGCCGGATCCAAGTCTGTGGAAGATCCGGACCAAGCCACCACC CCTACAGACGTGCGGGATGTTGACCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203949 protein sequence
Red=Cloning site Green=Tags(s) MELLCCEVDPVRRAVPDRNLLEDRVLQNLLTIEERYLPQCSYFKCVQKDIQPYMRRMVATWMLEVCEEQK CEEEVFPLAMNYLDRFLAGVPTPKTHLQLLGAVCMFLASKLKETIPLTAEKLCIYTDNSVKPQELLEWEL VVLGKLKWNLAAVTPHDFIEHILRKLPQQKEKLSLIRKHAQTFIALCATDFKFAMYPPSMIATGSVGAAI CGLQQDDEVNTLTCDALTELLAKITHTDVDCLKACQEQIEALLLNSLQQFRQEQHNAGSKSVEDPDQATT PTDVRDVDL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009829 |
| ORF Size | 870 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009829.3, NP_033959.1 |
| RefSeq Size | 5772 bp |
| RefSeq ORF | 870 bp |
| Locus ID | 12444 |
| UniProt ID | P30280 |
| MW | 32.9 kDa |
| Gene Summary | Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG203949 | Ccnd2 (tGFP-tagged) - Mouse cyclin D2 (Ccnd2) |
CNY 2,850.00 |
|
| MR203949L3 | Lenti ORF clone of Ccnd2 (Myc-DDK-tagged) - Mouse cyclin D2 (Ccnd2) |
CNY 4,750.00 |
|
| MR203949L4 | Lenti ORF clone of Ccnd2 (mGFP-tagged) - Mouse cyclin D2 (Ccnd2) |
CNY 4,750.00 |
