Abraxas1 (NM_172405) Mouse Tagged ORF Clone
CAT#: MR204136
- TrueORF®
Fam175a (Myc-DDK-tagged) - Mouse family with sequence similarity 175, member A (Fam175a)
"NM_172405" in other vectors (3)
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CNY 2,400.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 3830405G04Rik; 5630400M01Rik; AI506069; AL024423; AV118690; Ccdc98 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR204136 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACATTTAGAGAGCAGTTACTCCACAGAAACTTACAGACACATCTTTCAAGCCCTGAGCTGGTTTTCC TGTTGTTAACACCGAGTATAACAACAGAAAGCTGCTCCACTCACTGCCTGGAACACGCCTTATATAAACC TCAAAGGGGGCTTTTTCATAGGGTACCATTGGTGGTTACCAATCTGGGAATGTCTGATCAACTGGGTTAT AAAACAGAACCTGCTTCCTGTACATCCACTGTCTTTAGTAGAGCTGTGAGGACACACAGTTCTCAGTTCT TTAATGAAGATGGATCATTAAAGGAAGTCCATAAGATAAATGAAATGTATGCTGCTGTACAAGAAGAGTT AAAGAGTATATGCCAAAAAGTTGAACAAAGTGAACGAGAAGTAGAGAAACTATTGATGGATGTAAACCAA TTAAAAGAAGTTAGAAGAACCCAGCAAGCACGGGCTACAGGAGCAGGAGAGAAGAATGTCCAGAGAAACC CACAGGAGAACATCCTTCTTTGTCAAGCTTTGCGAACATTTTTTCCAGAGTCTGAAGTTCTTCATTCTTG CGTTATTTCTTTGAAAAATAGACACATTTCTCCCAGTGGGTGTAACGTTAACCACCATGTTGATGTAGTG GATCAGCTGACCTTAATGGTAGAGTATGTGTACAGCCCTGAAGCTAGTCCAGTGCCCACAGCCCAGCTAC GTAAACGGAAGGCTCTAGACACACAGGACCAGGGGTCTGTGAAGAGACCACGGCTGCTGGAGACAGAAAG CAGACCTTCTGTGGCAGCGTCCCGCAGCAGGCACCAAGACAAGGCTTCCTCAAGCAGCCTAGATATAGAC ATAGAGATGGGGAGCCCAGAAGATGATGCTGACTATCCTCGCTCTCCCACATTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204136 protein sequence
Red=Cloning site Green=Tags(s) MTFREQLLHRNLQTHLSSPELVFLLLTPSITTESCSTHCLEHALYKPQRGLFHRVPLVVTNLGMSDQLGY KTEPASCTSTVFSRAVRTHSSQFFNEDGSLKEVHKINEMYAAVQEELKSICQKVEQSEREVEKLLMDVNQ LKEVRRTQQARATGAGEKNVQRNPQENILLCQALRTFFPESEVLHSCVISLKNRHISPSGCNVNHHVDVV DQLTLMVEYVYSPEASPVPTAQLRKRKALDTQDQGSVKRPRLLETESRPSVAASRSRHQDKASSSSLDID IEMGSPEDDADYPRSPTF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_172405 |
ORF Size | 897 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_172405.1, NM_172405.2, NM_172405.3, NP_765993.1 |
RefSeq Size | 2499 bp |
RefSeq ORF | 1224 bp |
Locus ID | 70681 |
UniProt ID | Q8BPZ8 |
MW | 33.7 kDa |
Gene Summary | Involved in DNA damage response and double-strand break (DSB) repair. Component of the BRCA1-A complex, acting as a central scaffold protein that assembles the various components of the complex and mediates the recruitment of BRCA1. The BRCA1-A complex specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesion sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at DSBs. This complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC211092 | Fam175a (untagged) - Mouse family with sequence similarity 175, member A (Fam175a), (10ug) |
CNY 3,990.00 |
|
MR204136L3 | Lenti ORF clone of Fam175a (Myc-DDK-tagged) - Mouse family with sequence similarity 175, member A (Fam175a) |
CNY 4,750.00 |
|
MR204136L4 | Lenti ORF clone of Fam175a (mGFP-tagged) - Mouse family with sequence similarity 175, member A (Fam175a) |
CNY 4,750.00 |
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