Pard6b (BC025147) Mouse Tagged ORF Clone
CAT#: MR204154
- TrueORF®
Pard6b (Myc-DDK-tagged) - Mouse par-6 (partitioning defective 6) homolog beta (C
ORF Plasmid: tGFP
"BC025147" in other vectors (4)
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CNY 2,400.00
CNY 6,365.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AV025615; Par6b |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR204154 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACCGCGGCCACCGGCACGGGGCGAGCAGCGGCTGGCTGGGCACCATGGAGGTGAAGAGCAAGTTTG GAGCTGAGTTTCGTCGGTTTTCACTGGAAAGATCTAAGCCTGGAAAATTTGAGGAGTTTTACGGACTGCT GCAACATGTTCACAAGATCCCCAATGTCGACGTGTTGGTGGGCTACGCAGATATCCACGGGGACCTGCTG CCTATAAATAATGATGACAACTACCACAAGGCGGTTTCCACCGCCAATCCACTGCTCAGGATTTTTATAC AGAAGAAGGAAGAAGCTGACTACAGTGCCTTTGGCACCGACACCCTGATCAGGAAGAAGAACATGCTGAG CAACGTGCTGCGTCCCGACAACCACCGGAAGAAGCCTCACATCGTCATCAGCATGCCGCAGGACTTCCGG CCCGTGTCATCCATCATCGACGTGGACATCCTCCCAGAGACGCACCGGAGGGTCCGTCTGTACAAGTACG GCACAGAGAAGCCGCTGGGCTTCTACATCCGGGATGGCTCCAGCGTCCGAGTGACACCGCACGGCTTGGA GAAAGTCCCCGGCATCTTCATATCTCGGCTCGTCCCTGGGGGTCTGGCCCAGAGCACGGGCCTGCTAGCT GTCAACGATGAGGTGTTAGAAGTCAACGGTATAGAAGTGTCCGGGAAGAGCCTGGACCAGGTGACTGACA TGATGATAGCCAACAGCCGCAACCTCATCATCACCGTGCGCCCGGCCAACCAGAGGAACAACGTGGTGCG CAACAGTCGGACTTCTGGCAGCTCCAGCCAGTCCACTGACAACAGCCTCCTGGGCTTCCCACAGCAGGTG GAGGCCAGCTTCGAGCCCGAGGACCAGGACAGCGACGAGGACGACATCATCTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204154 protein sequence
Red=Cloning site Green=Tags(s) MNRGHRHGASSGWLGTMEVKSKFGAEFRRFSLERSKPGKFEEFYGLLQHVHKIPNVDVLVGYADIHGDLL PINNDDNYHKAVSTANPLLRIFIQKKEEADYSAFGTDTLIRKKNMLSNVLRPDNHRKKPHIVISMPQDFR PVSSIIDVDILPETHRRVRLYKYGTEKPLGFYIRDGSSVRVTPHGLEKVPGIFISRLVPGGLAQSTGLLA VNDEVLEVNGIEVSGKSLDQVTDMMIANSRNLIITVRPANQRNNVVRNSRTSGSSSQSTDNSLLGFPQQV EASFEPEDQDSDEDDIIC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | BC025147 |
ORF Size | 894 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | BC025147, AAH25147 |
RefSeq Size | 2705 bp |
RefSeq ORF | 896 bp |
Locus ID | 58220 |
MW | 33.3 kDa |
Gene Summary | Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC217807 | Pard6b (untagged) - Mouse par-6 (partitioning defective 6) homolog beta (C, (10ug) |
CNY 6,940.00 |
|
MG204154 | Pard6b (tGFP-tagged) - Mouse par-6 (partitioning defective 6) homolog beta (C |
CNY 2,850.00 |
|
MR204154L3 | Lenti ORF clone of Pard6b (Myc-DDK-tagged) - Mouse par-6 (partitioning defective 6) homolog beta (C |
CNY 4,750.00 |
|
MR204154L4 | Lenti ORF clone of Pard6b (mGFP-tagged) - Mouse par-6 (partitioning defective 6) homolog beta (C |
CNY 4,750.00 |