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Aqp4 (BC024526) Mouse Tagged ORF Clone
CAT＃: MR204192
- TrueORF®
Aqp4 (Myc-DDK-tagged) - Mouse aquaporin 4 (cDNA clone MGC:37514 IMAGE:4985265)
ORF Plasmid: tGFP
"BC024526" in other vectors (4)
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CNY 2,400.00
CNY 6,365.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MIWC, mMIWC |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR204192 representing BC024526
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGCTTTCAAAGGAGTCTGGACTCAGGCTTTCTGGAAGGCAGTCTCAGCAGAATTTCTGGCCACGC TTATCTTTGTTTTGCTCGGTGTGGGATCCACCATAAACTGGGGTGGCTCAGAAAACCCCTTACCTGTGGA CATGGTCCTCATCTCCCTTTGCTTTGGACTCAGCATTGCTACCATGGTGCAGTGCTTTGGCCACATCAGT GGTGGCCACATCAATCCCGCTGTGACTGTAGCCATGGTGTGCACACGAAAGATCAGCATCGCTAAGTCCG TCTTCTACATCATTGCACAGTGCCTGGGGGCCATCATTGGAGCCGGCATCCTCTACCTGGTCACACCTCC CAGTGTGGTTGGAGGATTGGGAGTCACCACGGTTCATGGAAACCTCACCGCTGGCCATGGGCTCCTGGTG GAGTTAATAATCACTTTCCAGTTGGTGTTCACTATTTTTGCCAGCTGTGATTCCAAACGAACTGATGTTA CTGGTTCAATAGCTTTAGCAATTGGATTTTCCGTTGCAATTGGACATTTGTTTGCAATCAATTATACTGG AGCCAGCATGAATCCAGCTCGATCTTTTGGACCCGCAGTTATCATGGGAAACTGGGCAAACCACTGGATA TATTGGGTTGGACCAATCATGGGCGCTGTGCTGGCAGGTGCCCTTTATGAGTATGTCTTCTGTCCTGATG TGGAGCTCAAACGTCGCCTTAAGGAAGCCTTCAGCAAAGCCGCGCAGCAGACAAAAGGGAGCTACATGGA GGTGGAGGACAACCGGAGCCAAGTGGAGACGGAAGACTTGATCCTGAAGCCCGGAGTGGTGCATGTGATT GACATTGACCGTGGAGAAGAGAAGAAGGGGAAAGACTCTTCGGGAGAGGTATTGTCTTCCGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204192 representing BC024526
Red=Cloning site Green=Tags(s) MVAFKGVWTQAFWKAVSAEFLATLIFVLLGVGSTINWGGSENPLPVDMVLISLCFGLSIATMVQCFGHIS GGHINPAVTVAMVCTRKISIAKSVFYIIAQCLGAIIGAGILYLVTPPSVVGGLGVTTVHGNLTAGHGLLV ELIITFQLVFTIFASCDSKRTDVTGSIALAIGFSVAIGHLFAINYTGASMNPARSFGPAVIMGNWANHWI YWVGPIMGAVLAGALYEYVFCPDVELKRRLKEAFSKAAQQTKGSYMEVEDNRSQVETEDLILKPGVVHVI DIDRGEEKKGKDSSGEVLSSV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC024526 |
| ORF Size | 903 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC024526, AAH24526 |
| RefSeq Size | 1622 bp |
| RefSeq ORF | 905 bp |
| Locus ID | 11829 |
| MW | 59.4 kDa |
| Gene Summary | This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. This protein is the predominant aquaporin found in brain and has an important role in brain water homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Dec 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217687 | Aqp4 (untagged) - Mouse aquaporin 4 (cDNA clone MGC:37514 IMAGE:4985265), (10ug) |
CNY 6,940.00 |
|
| MG204192 | Aqp4 (tGFP-tagged) - Mouse aquaporin 4 (cDNA clone MGC:37514 IMAGE:4985265) |
CNY 4,000.00 |
|
| MR204192L3 | Lenti ORF clone of Aqp4 (Myc-DDK-tagged) - Mouse aquaporin 4 (cDNA clone MGC:37514 IMAGE:4985265) |
CNY 4,750.00 |
|
| MR204192L4 | Lenti ORF clone of Aqp4 (mGFP-tagged) - Mouse aquaporin 4 (cDNA clone MGC:37514 IMAGE:4985265) |
CNY 4,800.00 |
