Klf7 (NM_033563) Mouse Tagged ORF Clone
CAT#: MR204201
- TrueORF®
Klf7 (Myc-DDK-tagged) - Mouse Kruppel-like factor 7 (ubiquitous) (Klf7)
ORF Plasmid: tGFP
"NM_033563" in other vectors (4)
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CNY 2,400.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 9830124P08Rik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR204201 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGTGTTGGCTAGTTATAGTATATTCCAGGAGCTACAACTTGTCCACGACACCGGCTACTTCTCAG CTTTGCCATCCCTGGAGGAGACCTGGCAGCAGACATGCCTTGAGTTGGAACGCTACCTCCAGACAGAACC CCGGCGGATCTCGGAGACCTTTGGTGAGGACTTGGACTGCTTCCTCCACGCTTCTCCTCCCCCATGCATT GAGGAGAGCTTCCGGCGCCTAGACCCCTTGCTGCTCCCAGTGGAAGCCACCATCTGCGAGAAGAGCTCCG CAGTGGACATTTTGCTCTCTCGGGACAAGTTGCTATCTGAGACCTGCCTCAGCCTCCAGCCAACCAGCTC TTCTCTAGACAGCTACACAGCCGTCAACCAGGCCCAGCTCAACGCAGTGACCTCATTAACGCCGCCCTCA TCCCCTGAGCTCAGCCGCCATCTGGTCAAAACCTCACAGACTCTCTCAGCCGTGGATGGCACAGTGACGT TGAAACTGGTGGCCAAGAAGGCTTCCCTCAGCTCGGTGAAGGTGGGAGGGGTGGCGGCGGCGGCAGCCGT GACACCAGCTGGAGCAGTTAAGAGTGGACAGAGCGACAGTGAGCAAGGAGGGGGAGGGGCTGACACATGC CCTGAAAACAAGAAGAGGGTCCATCGCTGTCAGTTTAACGGGTGCCGGAAAGTTTATACAAAAAGCTCCC ACTTAAAGGCCCACCAGAGGACTCACACAGGTGAGAAGCCTTACAAGTGCTCATGGGAAGGATGCGAGTG GCGTTTTGCACGGAGCGATGAGCTCACAAGGCACTACAGGAAACACACAGGTGCAAAGCCCTTTAAGTGT AACCACTGCGACAGGTGTTTCTCCAGGTCTGACCATCTTGCGCTCCATATGAAGAGACATATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204201 protein sequence
Red=Cloning site Green=Tags(s) MDVLASYSIFQELQLVHDTGYFSALPSLEETWQQTCLELERYLQTEPRRISETFGEDLDCFLHASPPPCI EESFRRLDPLLLPVEATICEKSSAVDILLSRDKLLSETCLSLQPTSSSLDSYTAVNQAQLNAVTSLTPPS SPELSRHLVKTSQTLSAVDGTVTLKLVAKKASLSSVKVGGVAAAAAVTPAGAVKSGQSDSEQGGGGADTC PENKKRVHRCQFNGCRKVYTKSSHLKAHQRTHTGEKPYKCSWEGCEWRFARSDELTRHYRKHTGAKPFKC NHCDRCFSRSDHLALHMKRHI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_033563 |
ORF Size | 906 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_033563.2, NP_291041.2 |
RefSeq Size | 1344 bp |
RefSeq ORF | 906 bp |
Locus ID | 93691 |
UniProt ID | Q99JB0 |
MW | 33.3 kDa |
Gene Summary | Transcriptional factor (PubMed:11245580). Plays a critical role in neuronal morphogenesis (PubMed:11336497, PubMed:15964824). Represses the corneal epithelium differentiation (By similarity). Acts also as a metabolic regulator, by modulating insulin sensitivity in pancreatic beta cells and skeletal muscle cells. Inhibits transcriptional inducers of adipogenesis and has a repressive role in the expression of several adipokines, including leptin (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC205347 | Klf7 (untagged) - Mouse Kruppel-like factor 7 (ubiquitous) (Klf7), (10ug) |
CNY 2,400.00 |
|
MG204201 | Klf7 (tGFP-tagged) - Mouse Kruppel-like factor 7 (ubiquitous) (Klf7) |
CNY 2,850.00 |
|
MR204201L3 | Lenti ORF clone of Klf7 (Myc-DDK-tagged) - Mouse Kruppel-like factor 7 (ubiquitous) (Klf7) |
CNY 4,750.00 |
|
MR204201L4 | Lenti ORF clone of Klf7 (mGFP-tagged) - Mouse Kruppel-like factor 7 (ubiquitous) (Klf7) |
CNY 4,750.00 |