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Nek7 (NM_021605) Mouse Tagged ORF Clone
CAT＃: MR204231
- TrueORF®
Nek7 (Myc-DDK-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7)
ORF Plasmid: tGFP
"NM_021605" in other vectors (4)
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CNY 2,081.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2810460C19Rik; AU020186 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR204231 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGAACAATCACAAGGAATGCAAGGGCCGCCCGTTACTCAGTTCCAGCCACAGAAGGCATTACGGC CAGATATGGGCTATAATACTTTAGCCAACTTCCGAATAGAAAAGAAAATTGGTCGTGGACAATTTAGTGA AGTTTATAGAGCATCCTGTCTCTTGGATGGAGTGCCGGTAGCGTTAAAAAAAGTACAGATATTTGATTTA ATGGATGCCAAAGCACGTGCTGATTGTATCAAAGAAATAGACCTCCTTAAGCAACTCAACCATCCAAATG TAATTAAATACTATGCATCATTCATTGAGGATAATGAGCTGAACATAGTTTTGGAGTTAGCAGATGCTGG TGACCTCTCCAGAATGATAAAGCACTTTAAGAAACAAAAGAGGCTAATCCCTGAGAGAACCGTTTGGAAA TACTTCGTTCAGCTCTGCAGTGCACTGGACCACATGCATTCTCGAAGAGTCATGCACAGAGATATAAAAC CAGCTAATGTGTTCATTACAGCCACTGGGGTAGTAAAACTCGGAGACCTTGGGCTTGGTCGGTTTTTCAG CTCCAAAACCACAGCTGCACATTCTTTAGTGGGTACACCTTACTACATGTCTCCAGAGAGAATACATGAA AATGGATACAACTTCAAGTCTGACATCTGGTCTCTTGGCTGTCTGCTATATGAGATGGCTGCACTGCAGA GTCCTTTCTACGGCGACAAGATGAACTTGTATTCTCTGTGTAAGAAGATAGAGCAGTGTGACTACCCGCC TCTCCCGTCAGATCACTATTCGGAGGAGCTACGACAGCTAGTTAATATATGCATCAACCCAGATCCAGAG AAGCGACCCGACATCGCCTATGTTTATGATGTGGCAAAGAGGATGCATGCATGTACCGCAAGCACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204231 protein sequence
Red=Cloning site Green=Tags(s) MDEQSQGMQGPPVTQFQPQKALRPDMGYNTLANFRIEKKIGRGQFSEVYRASCLLDGVPVALKKVQIFDL MDAKARADCIKEIDLLKQLNHPNVIKYYASFIEDNELNIVLELADAGDLSRMIKHFKKQKRLIPERTVWK YFVQLCSALDHMHSRRVMHRDIKPANVFITATGVVKLGDLGLGRFFSSKTTAAHSLVGTPYYMSPERIHE NGYNFKSDIWSLGCLLYEMAALQSPFYGDKMNLYSLCKKIEQCDYPPLPSDHYSEELRQLVNICINPDPE KRPDIAYVYDVAKRMHACTAST TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021605 |
| ORF Size | 909 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021605.4 |
| RefSeq Size | 3366 bp |
| RefSeq ORF | 909 bp |
| Locus ID | 59125 |
| UniProt ID | Q9ES74 |
| MW | 34.5 kDa |
| Gene Summary | Protein kinase which plays an important role in mitotic cell cycle progression. Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. Phosphorylates RPS6KB1.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203059 | Nek7 (untagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7), (10ug) |
CNY 2,000.00 |
|
| MG204231 | Nek7 (tGFP-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7) |
CNY 2,850.00 |
|
| MR204231L3 | Lenti ORF clone of Nek7 (Myc-DDK-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7) |
CNY 4,750.00 |
|
| MR204231L4 | Lenti ORF clone of Nek7 (mGFP-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7) |
CNY 4,750.00 |
