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Abraxas2 (NM_198017) Mouse Tagged ORF Clone
CAT＃: MR204413
- TrueORF®
Fam175b (Myc-DDK-tagged) - Mouse family with sequence similarity 175, member B (Fam175b)
ORF Plasmid: tGFP
"NM_198017" in other vectors (3)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AA589499; Abro1; AI853413; C430003P19Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR204413 representing NM_198017
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGGGGAGGTGGAGATTTCCATCATGGCGGCTTCCATTTCGGGCTACACCTTCAGCGCTGTGTGTT TCCACAGCGCCAACAGCAACGCCGACCACGAAGGATTTTTGCTGGGAGAGGTAAGACAAGAGGAAACTTT TAGCATCAGTGACTCACAAATCAGCAACACAGAGTTTCTGCAAGTAATTGAGATCCATAACCATCAGCCT TGTTCACAACTCTTCAGTTTTTATGATTATGCGAGCAAAGTGAATGAAGAAAGTTTGGACAGAATTCTGA AAGATCGGAGGAAGAAAGTTATCGGATGGTACAGGTTCCGGCGAAACACCCAGCAGCAGATGTCCTACAG GGAACAGGTCATCCACAAGCAGCTCACCCGCATCCTCGGCGTGCCAGACCTGGTCTTCCTTCTCTTCAGC TTCATCTCTACCGCCAACAATTCTACTCATGCTTTAGAATATGTTCTCTTTAGACCAAACCGCAGGTATA ATCAAAGGATATCACTTGCTATTCCCAATCTAGGCAATACTAGCCAGCAAGAGTACAAAGTGTCTTCAGT GCCAAACACGTCTCAGAGTTATGCCAAGGTCATTAAAGAGCACGGTACTGACTTTTTTGACAAGGATGGA GTCATGAAGGACATCAGGGCAATTTATCAGGTTTATAATGCACTTCAGGAGAAAGTACAGGCAGTATGTG CAGATGTAGAAAAGAGTGAGCGAGTTGTTGAATCTTGTCAGGCAGAAGTGAACAAATTGAGAAGACAAAT CACTCAGAAGAAAAATGAAAAGGAACAAGAAAGAAGATTGCAGCAGGCCCTGTTAAGCAGACAGATGCCA TCCGAAAGTCTGGAGCCAGCGTTCAGCCCTCGGATGTCCTATTCTGGGTTTTCAGCTGAAGGGAGAAGTA CTCTGGCAGAAACGGAGCCCTCTGATCCTCCGCCCCCTTACTCTGATTTCCACCCAAACAATCAAGAAAG TACTCTGAGCCATTCTCGCATGGAAAGGAGTGTCTTCATGCCCCGTCCTCAGGCTGTGGGCTCTTCCAGC TATGCTTCCACCAGTGGCGGACTGAAGTTCACTGGAAGTGGAGCAGATCTTCTTCCTTCCCAGAGTGCAG CTGGAGACAGTGGGGAGGAGTCAGATGACAGTGATTATGAAAATTTGATTGATCCTGCAGAGTCCCCTCA TAGCGAATACTCACATTCAAAGAATTCACGGCCCTCGACACATCCTGACGAGGACCCCAGGAACACTCAG ACCTCCCAGATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204413 representing NM_198017
Red=Cloning site Green=Tags(s) MMGEVEISIMAASISGYTFSAVCFHSANSNADHEGFLLGEVRQEETFSISDSQISNTEFLQVIEIHNHQP CSQLFSFYDYASKVNEESLDRILKDRRKKVIGWYRFRRNTQQQMSYREQVIHKQLTRILGVPDLVFLLFS FISTANNSTHALEYVLFRPNRRYNQRISLAIPNLGNTSQQEYKVSSVPNTSQSYAKVIKEHGTDFFDKDG VMKDIRAIYQVYNALQEKVQAVCADVEKSERVVESCQAEVNKLRRQITQKKNEKEQERRLQQALLSRQMP SESLEPAFSPRMSYSGFSAEGRSTLAETEPSDPPPPYSDFHPNNQESTLSHSRMERSVFMPRPQAVGSSS YASTSGGLKFTGSGADLLPSQSAAGDSGEESDDSDYENLIDPAESPHSEYSHSKNSRPSTHPDEDPRNTQ TSQI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_198017 |
| ORF Size | 1272 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_198017.3, NP_932134.3 |
| RefSeq Size | 2895 bp |
| RefSeq ORF | 1275 bp |
| Locus ID | 109359 |
| UniProt ID | Q3TCJ1 |
| MW | 47.9 kDa |
| Gene Summary | Component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked polyubiquitin, leaving the last ubiquitin chain attached to its substrates. May act as a central scaffold protein that assembles the various components of the BRISC complex and retains them in the cytoplasm (By similarity). Plays a role in regulating the onset of apoptosis via its role in modulating 'Lys-63'-linked ubiquitination of target proteins (PubMed:21195082). Required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1. Plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1; deubiquitination increases IFNAR1 activities by enhancing its stability and cell surface expression (PubMed:24075985, PubMed:26344097). Down-regulates the response to bacterial lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination (PubMed:24075985). Required for normal induction of p53/TP53 in response to DNA damage. Independent of the BRISC complex, promotes interaction between USP7 and p53/TP53, and thereby promotes deubiquitination of p53/TP53, preventing its degradation and resulting in increased p53/TP53-mediated transcription regulation and p53/TP53-dependent apoptosis in response to DNA damage (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG204413 | Fam175b (tGFP-tagged) - Mouse RIKEN cDNA C430003P19 gene (C430003P19Rik) |
CNY 3,710.00 |
|
| MR204413L3 | Lenti ORF clone of Fam175b (Myc-DDK-tagged) - Mouse family with sequence similarity 175, member B (Fam175b) |
CNY 5,230.00 |
|
| MR204413L4 | Lenti ORF clone of Fam175b (mGFP-tagged) - Mouse family with sequence similarity 175, member B (Fam175b) |
CNY 5,230.00 |
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