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Krt13 (BC060286) Mouse Tagged ORF Clone
CAT＃: MR204414
- TrueORF®
Krt13 (Myc-DDK-tagged) - Mouse keratin 13 (cDNA clone MGC:70026 IMAGE:30292772)
ORF Plasmid: tGFP
"BC060286" in other vectors (4)
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CNY 3,140.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CK13, K13 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR204414 representing BC060286
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTGCCGCTTCCAGAGTTCCTCCATGAGCTATGGAGGTGGTTTCGGAGCTGGTTCTTGCCAGCTTG GAGGAGGCCGTAATATCTCCTCCTGCTCATCTCGGTTTGTCACTGGAGGGTCAGCTGGAGGCTATGGAGG GGGCATGAGCTGTGGCTTTGGTGGAGGAGCTGGTGGTGGCTTTGGAGGTGGCTTTGGAGGTGGCTTTGGA GGTAGCTATGGCGGAGGTTTTGGCGGTGGCTTTGGTGACTTCGGAGGTGTCGATGGTGGCCTCCTCTCTG GCAACGAGAAGATCACCATGCAGAACCTCAATGACCGCCTGGCCAGCTACCTGGATAAGGTGCGCGCCTT GGAGGCAGCCAATGCCGACCTGGAGGTGAAGATTCGTGACTGGCATCTGAAACAGAGCCCAGCTAGTCCA GAGCGGGACTACAGTGCTTATTATAAGACCATCGAGGAGCTCCGGATCAAGATTCTGGAAGCCACCACGG ACAACAACCGGATCATCCTGGAGATTGACAATGCCAGGCTGGCTGCAGATGACTTCAGGCTCAAGTACGA AAATGAGCTGACCTTGCGCCAGAGCGTGGAGGCCGACATCAACGGCCTGCGCAGGGTGCTGGATGAGCTG ACTCTGGCTAAGACTGACCTGGAAATGCAGATTGAGAGCCTGAATGAAGAACTGGCTTACCTGAAGAAGA ACCACGAAGAGGAGATGAAGGAATTCAGCAACCAGGTAGTAGGCCAGGTCAATGTGGAGATGGACGCCAC CCCTGGCATTGATCTGACCCGTGTGCTGGCTGAGATGAGGGAGCAGTATGAAGCCCTGGCAGAGAAGAAT CGGAGGGATGCTGAGGAATGGTTCCAGACCAAGAGTGCAGAGCTGAACAAGGAAGTATCCTCCAATTCCG GTCGCCCAGATTTCCGAAAGTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204414 representing BC060286
Red=Cloning site Green=Tags(s) MSCRFQSSSMSYGGGFGAGSCQLGGGRNISSCSSRFVTGGSAGGYGGGMSCGFGGGAGGGFGGGFGGGFG GSYGGGFGGGFGDFGGVDGGLLSGNEKITMQNLNDRLASYLDKVRALEAANADLEVKIRDWHLKQSPASP ERDYSAYYKTIEELRIKILEATTDNNRIILEIDNARLAADDFRLKYENELTLRQSVEADINGLRRVLDEL TLAKTDLEMQIESLNEELAYLKKNHEEEMKEFSNQVVGQVNVEMDATPGIDLTRVLAEMREQYEALAEKN RRDAEEWFQTKSAELNKEVSSNSGRPDFRKY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC060286 |
| ORF Size | 933 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC060286.1 |
| RefSeq Size | 1232 bp |
| RefSeq ORF | 935 bp |
| Locus ID | 16663 |
| MW | 45.1 kDa |
| Gene Summary | The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218195 | Krt13 (untagged) - Mouse keratin 13 (cDNA clone MGC:70026 IMAGE:30292772), (10ug) |
CNY 6,940.00 |
|
| MG204414 | Krt13 (tGFP-tagged) - Mouse keratin 13 (cDNA clone MGC:70026 IMAGE:30292772) |
CNY 3,420.00 |
|
| MR204414L3 | Lenti ORF clone of Krt13 (Myc-DDK-tagged) - Mouse keratin 13 (cDNA clone MGC:70026 IMAGE:30292772) |
CNY 5,040.00 |
|
| MR204414L4 | Lenti ORF clone of Krt13 (mGFP-tagged) - Mouse keratin 13 (cDNA clone MGC:70026 IMAGE:30292772) |
CNY 5,040.00 |
