Plscr1 (NM_011636) Mouse Tagged ORF Clone

CAT#: MR204796

  • TrueORF®

Plscr1 (Myc-DDK-tagged) - Mouse phospholipid scramblase 1 (Plscr1)



  "NM_011636" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 2,400.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms MmTRA1a; MmTRA1b; Nor1; Tra1; Tra1a; Tra1b; Tras1; Tras2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR204796 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAAACCACAGCAAGCAAACTGAGGCTCCCCACCCGGGAACATATATGCCAGCTGGGTATCCCCCTC
CGTATCCACCAGCAGCTTTCCAAGGACCTTCAGACCATGCTGCTTACCCCATACCCCAGGCTGGCTACCA
AGGGCCTCCGGGCCCCTATCCAGGGCCCCAACCTGGCTACCCAGTCCCACCAGGAGGTTATGCAGGTGGT
GGCCCTAGTGGCTTTCCTGTCCAAAATCAGCCAGCATATAATCATCCAGGTGGGCCTGGGGGGACCCCAT
GGATGCCAGCCCCCCCACCTCCACTGAACTGTCCACCGGGGCTGGAATACTTAGCTCAGATTGATCAGCT
TCTGGTTCATCAGCAAATTGAGCTTCTGGAAGTCTTAACAGGCTTTGAAACAAATAACAAATATGAAATC
AAGAACAGCCTCGGGCAGAGAGTTTACTTTGCAGTGGAAGATACTGACTGCTGTACCCGAAACTGCTGTG
GGGCGTCTAGACCTTTCACCTTGAGGATCCTGGATAATCTGGGCCGAGAAGTCATGACTCTGGAGCGACC
TCTGAGATGCAGTAGCTGCTGCTTCCCCTGCTGCCTCCAGGAGATAGAAATCCAGGCTCCTCCTGGGGTG
CCAGTAGGTTATGTGACTCAGACCTGGCACCCATGTCTGCCCAAGTTCACTCTCCAAAATGAGAAGAAGC
AGGATGTCCTGAAAGTAGTTGGTCCGTGTGTTGTGTGTAGCTGCTGTTCCGACATTGACTTTGAGCTCAA
ATCTCTAGATGAAGAATCAGTAGTTGGCAAAATTTCTAAGCAGTGGTCTGGTTTTGTGAGAGAGGCCTTC
ACGGATGCAGACAACTTTGGGATCCAGTTCCCGCTAGACCTGGATGTGAAGATGAAAGCTGTGATGCTTG
GTGCTTGTTTCCTCATAGATTTCATGTTTTTTGAAAGAACTGGAAACGAGGAACAAAGATCAGGAGTATG
G


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR204796 protein sequence
Red=Cloning site Green=Tags(s)

MENHSKQTEAPHPGTYMPAGYPPPYPPAAFQGPSDHAAYPIPQAGYQGPPGPYPGPQPGYPVPPGGYAGG
GPSGFPVQNQPAYNHPGGPGGTPWMPAPPPPLNCPPGLEYLAQIDQLLVHQQIELLEVLTGFETNNKYEI
KNSLGQRVYFAVEDTDCCTRNCCGASRPFTLRILDNLGREVMTLERPLRCSSCCFPCCLQEIEIQAPPGV
PVGYVTQTWHPCLPKFTLQNEKKQDVLKVVGPCVVCSCCSDIDFELKSLDEESVVGKISKQWSGFVREAF
TDADNFGIQFPLDLDVKMKAVMLGACFLIDFMFFERTGNEEQRSGVW

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_011636
ORF Size 984 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_011636.2, NP_035766.2
RefSeq Size 2141 bp
RefSeq ORF 987 bp
Locus ID 22038
UniProt ID Q9JJ00
MW 35.8 kDa
Gene Summary May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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