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Trib3 (BC012955) Mouse Tagged ORF Clone
CAT＃: MR204806
- TrueORF®
Trib3 (Myc-DDK-tagged) - Mouse tribbles homolog 3 (Drosophila) (cDNA clone MGC:18731 IMAGE:3980838)
ORF Plasmid: tGFP
"BC012955" in other vectors (4)
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CNY 2,400.00
CNY 6,365.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Trb3, Nipk, SINK, SKIP3, TRB-3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR204806 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGAGCTACACCTCTGGCTGCTTCTGCTGATGTTTCCTGCAGGAAGAAACCGTTGGAGTTTGATGACA ATATTGATGCCAAGTGTCCAGTCCTAAAACGAGTGAGAGATGAGCCTGAGCCCGGACCACTCCCCAGCCT GCTGCCCCCCAGCCCACCTCCCGCCTCAGACTTGTCACCTGCTGTGGCCCCTGCAACTCGACTGGGGCCT TATATCCTTTTGGAACGAGAGCAAGGCAGCTGCAGCTATCGAGCCCTGCACTGCCCCACAGGCACAGAGT ACACCTGCAAGGTGTACCCTGCCAGCGAGGCCCAGGCGGTGCTGGCACCTTATGCCCGGCTGCCTACCCA CCAGCATGTGGCCCGTCCCACAGAGGTCCTGCTGGGCTCTCGGCTCCTTTACATCTTTTTCACGAAGACC CATGGGGACTTGCACAGCCTGGTGCGCAGCCGCCGCGGTATCCCAGAGCCCGAGGCTGCCGGGCTCTTCC GGCAGATGGCTAGTGCCGTGGCACACTGCCACAAGCACGGGCTTGTCTTGCGCGACCTCAAGCTGCGTCG CTTTGTCTTCAGCAACTGTGAGAGGACGAAGCTGGTGCTGGAGAACCTGGAAGATGCCTGCGTGATGACT GGATCAGATGACTCTCTGTGGGACAAGCATGCGTGCCCTGCCTACGTGGGACCAGAGATACTCAGCTCCC GGCCATCCTACTCTGAGCCAGTCCTGCTCTTTGGCAAGATCCGTAGAGGGACCTTTGCCCTGCCTGAGGG CCTATCAGCCCCAGCCCGCTGTCTGATCCGCTGTCTCCTCCGCAAGGAACCTTCAGAGCGACTTGTGGCC CTGGGAATCCTCTTGCATCCCTGGTTGAGAGAGGATCACGGCCGAGTCTCTCCTCCACAGTCTGACCGAA GGGAGATGGACCAGGTGGTCCCAGATGGGCCACAGCTGGAGGAGGCTGAGGAAGGGGAGGTGGGGCTGTA CGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204806 protein sequence
Red=Cloning site Green=Tags(s) MRATPLAASADVSCRKKPLEFDDNIDAKCPVLKRVRDEPEPGPLPSLLPPSPPPASDLSPAVAPATRLGP YILLEREQGSCSYRALHCPTGTEYTCKVYPASEAQAVLAPYARLPTHQHVARPTEVLLGSRLLYIFFTKT HGDLHSLVRSRRGIPEPEAAGLFRQMASAVAHCHKHGLVLRDLKLRRFVFSNCERTKLVLENLEDACVMT GSDDSLWDKHACPAYVGPEILSSRPSYSEPVLLFGKIRRGTFALPEGLSAPARCLIRCLLRKEPSERLVA LGILLHPWLREDHGRVSPPQSDRREMDQVVPDGPQLEEAEEGEVGLYG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC012955 |
| ORF Size | 984 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC012955, AAH12955 |
| RefSeq Size | 1969 bp |
| RefSeq ORF | 986 bp |
| Locus ID | 228775 |
| MW | 36.2 kDa |
| Gene Summary | Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity. Inhibits the transcriptional activity of DDIT3/CHOP and is involved in DDIT3/CHOP-dependent cell death during ER stress (By similarity). Can inhibit APOBEC3A editing of nuclear DNA.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Tribbles Homolog 3 Mediates the Development and Progression of Diabetic Retinopathy
,Pitale, PM;Saltykova, IV;Adu-Agyeiwaah, Y;Calzi, SL;Satoh, T;Akira, S;Gorbatyuk, O;Boulton, ME;Pardue, MT;Garvey, WT;Athar, M;Grant, MB;Gorbatyuk, MS;,
Diabetes
,PubMed ID 33975909
[TRIB3]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200945 | Trib3 (untagged) - Mouse tribbles homolog 3 (Drosophila) (cDNA clone MGC:18731 IMAGE:3980838), (10ug) |
CNY 2,400.00 |
|
| MG204806 | Trib3 (tGFP-tagged) - Mouse tribbles homolog 3 (Drosophila) (cDNA clone MGC:18731 IMAGE:3980838) |
CNY 2,850.00 |
|
| MR204806L3 | Lenti ORF clone of Trib3 (Myc-DDK-tagged) - Mouse tribbles homolog 3 (Drosophila) (cDNA clone MGC:18731 IMAGE:3980838) |
CNY 4,750.00 |
|
| MR204806L4 | Lenti ORF clone of Trib3 (mGFP-tagged) - Mouse tribbles homolog 3 (Drosophila) (cDNA clone MGC:18731 IMAGE:3980838) |
CNY 4,750.00 |
