Pbk (NM_023209) Mouse Tagged ORF Clone
CAT#: MR204854
- TrueORF®
Pbk (Myc-DDK-tagged) - Mouse PDZ binding kinase (Pbk)
ORF Plasmid: tGFP
"NM_023209" in other vectors (4)
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CNY 2,400.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2810434B10Rik; AW538537; D14Ertd732e; TOPK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR204854 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGGAATTAATAATTTCAAGACGCCAAACAAATCTGAAAAAAGGAAATCTGTATTATGTTCCACTC CATGTGTAAATATCCCTGCCTCTCCATTTATGCAGAAGCTTGGCTTTGGGACTGGGGTCAGCGTTTACCT AATGAAAAGATCTCCAAGAGGGTTGTCTCATTCTCCTTGGGCCGTGAAAAAGATAAGTCTTTTATGCGAT GATCATTATCGAACTGTGTATCAGAAGAGACTAACTGATGAAGCTAAGATTTTAAAAAACCTTAATCACC CAAACATTATAGGATATCGTGCTTTTACTGAAGCCAGTGATGGTAGTCTGTGCCTTGCTATGGAGTATGG AGGTGAAAAGTCTCTGAATGACTTAATAGAAGAGCGGAACAAAGACAGTGGAAGTCCTTTTCCAGCAGCT GTAATTCTCAGAGTTGCTTTGCACATGGCCAGAGGGCTAAAGTACCTGCACCAAGAAAAGAAGCTGCTTC ATGGAGACATAAAGTCTTCAAATGTTGTAATTAAAGGTGATTTTGAAACAATTAAAATCTGTGATGTAGG AGTCTCTCTGCCATTGGATGAAAATATGACTGTGACTGATCCTGAGGCCTGTTATATTGGTACTGAGCCA TGGAAACCCAAGGAAGCGTTGGAAGAAAATGGCATCATTACTGACAAGGCAGATGTGTTTGCTTTTGGCC TTACTCTGTGGGAAATGATGACTTTATGTATTCCACACGTCAATCTTCCAGATGATGATGTTGATGAAGA TGCAACCTTTGATGAGAGTGACTTCGATGATGAAGCATATTATGCAGCTCTGGGGACAAGGCCATCCATC AACATGGAAGAGCTGGATGACTCCTACCAGAAGGCCATTGAACTCTTCTGTGTGTGCACTAATGAGGATC CTAAAGATCGCCCGTCTGCTGCACACATCGTTGAAGCTTTGGAACTAGATGGCCAATGTTGTGGTCTAAG CTCAAAGCAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204854 protein sequence
Red=Cloning site Green=Tags(s) MEGINNFKTPNKSEKRKSVLCSTPCVNIPASPFMQKLGFGTGVSVYLMKRSPRGLSHSPWAVKKISLLCD DHYRTVYQKRLTDEAKILKNLNHPNIIGYRAFTEASDGSLCLAMEYGGEKSLNDLIEERNKDSGSPFPAA VILRVALHMARGLKYLHQEKKLLHGDIKSSNVVIKGDFETIKICDVGVSLPLDENMTVTDPEACYIGTEP WKPKEALEENGIITDKADVFAFGLTLWEMMTLCIPHVNLPDDDVDEDATFDESDFDDEAYYAALGTRPSI NMEELDDSYQKAIELFCVCTNEDPKDRPSAAHIVEALELDGQCCGLSSKH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_023209 |
ORF Size | 993 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_023209.2 |
RefSeq Size | 1663 bp |
RefSeq ORF | 993 bp |
Locus ID | 52033 |
UniProt ID | Q9JJ78 |
MW | 36.7 kDa |
Gene Summary | Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC201207 | Pbk (untagged) - Mouse PDZ binding kinase (Pbk), (10ug) |
CNY 2,000.00 |
|
MG204854 | Pbk (tGFP-tagged) - Mouse PDZ binding kinase (Pbk) |
CNY 2,850.00 |
|
MR204854L3 | Lenti ORF clone of Pbk (Myc-DDK-tagged) - Mouse PDZ binding kinase (Pbk) |
CNY 4,750.00 |
|
MR204854L4 | Lenti ORF clone of Pbk (mGFP-tagged) - Mouse PDZ binding kinase (Pbk) |
CNY 4,750.00 |