Tdp1 (BC019804) Mouse Tagged ORF Clone

CAT#: MR205151

  • TrueORF®

Tdp1 (Myc-DDK-tagged) - Mouse tyrosyl-DNA phosphodiesterase 1 (cDNA clone MGC:30935 IMAGE:4018534)

ORF Plasmid: DDK tGFP



  "BC019804" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,656.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 2810481F14Rik; 4921509N21Rik; AI838772; AW493413; E430034L06Rik; MLZ-501; SCAN1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR205151 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGCTCTTGCTCTATGAAGAAGGCCTCCGAGTTGTCATCCACACCTCCAACCTCATCCGGGAAGACT
GGCACCAGAAAACCCAAGGAATATGGTTGAGCCCTTTGTACCCGAGAATTGACCAGGGAAGCCACGCAGC
TGGAGAGTCAAGCACCCGTTTCAAAGCTGACCTCATCAGTTATCTGACGGCGTATAACGCTCCCCCTTTG
CAGGAGTGGATAGACATCATCCAAGAGCATGACCTCTCTGAGACCAATGTTTACCTCATTGGTTCAACCC
CTGGACGCTTTCAAGGAAGTCACAGAGATAACTGGGGACATTTTAGGCTTAGAAAGCTTCTGCAAGCGCA
TGCCCCGTCCACACCCAAGGGCGAGTGCTGGCCTATCGTGGGTCAGTTCTCAAGCATCGGCTCCCTGGGG
CCCGATGAATCCAAGTGGCTGTGCTCAGAGTTTAAAGACAGCTTGCTGGCACTGAGAGAGGAAGGCCGAC
CTCCGGGCAAGAGTGCAGTTCCTCTTCATCTGATCTATCCCTCTGTGGAAAATGTGCGGACCAGCTTAGA
AGGATACCCTGCTGGGGGCTCTCTTCCCTATAGCATCCAGACGGCTGAAAAGCAGAGGTGGCTGCACTCC
TACTTCCACAAGTGGTCAGCAGAGACATCCGGCCGAAGTAATGCCATGCCACATATTAAGACGTACATGA
GGCCCTCGCCAGACTTCAGTAAACTGGCTTGGTTCCTGGTCACAAGTGCGAATCTGTCCAAGGCTGCCTG
GGGGGCACTGGAGAAGAACGGCACCCAGCTGATGATACGCTCCTACGAGCTCGGGGTCCTCTTCCTCCCG
TCTGCGTTTGGCCTGGACACCTTCAAAGTGAAACAGAAATTCTTCTCCAGCAGCTGTGAGCCGACGGCCT
CCTTTCCTGTGCCCTATGACCTGCCCCCAGAGCTGTACAGGAGTAAAGACCGGCCTTGGATTTGGAACAT
TCCTTATGTCAAAGCACCTGACACGCACGGCAACATGTGGGTGCCCTCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR205151 protein sequence
Red=Cloning site Green=Tags(s)

MMLLLYEEGLRVVIHTSNLIREDWHQKTQGIWLSPLYPRIDQGSHAAGESSTRFKADLISYLTAYNAPPL
QEWIDIIQEHDLSETNVYLIGSTPGRFQGSHRDNWGHFRLRKLLQAHAPSTPKGECWPIVGQFSSIGSLG
PDESKWLCSEFKDSLLALREEGRPPGKSAVPLHLIYPSVENVRTSLEGYPAGGSLPYSIQTAEKQRWLHS
YFHKWSAETSGRSNAMPHIKTYMRPSPDFSKLAWFLVTSANLSKAAWGALEKNGTQLMIRSYELGVLFLP
SAFGLDTFKVKQKFFSSSCEPTASFPVPYDLPPELYRSKDRPWIWNIPYVKAPDTHGNMWVPS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC019804
ORF Size 1029 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC019804, AAH19804
RefSeq Size 1392 bp
RefSeq ORF 1031 bp
Locus ID 104884
MW 38.9 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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