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Tusc3 (NM_030254) Mouse Tagged ORF Clone
CAT＃: MR205219
- TrueORF®
Tusc3 (Myc-DDK-tagged) - Mouse tumor suppressor candidate 3 (Tusc3)
ORF Plasmid: tGFP
"NM_030254" in other vectors (4)
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CNY 3,330.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AU022242; BC003311; N33 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR205219 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGCCAGGGCCGCTCCTTCACGCCGGAGACAGGCGGGCCGCCGGTTGCGCTACCTGCCTACTGGTA GCTTTCCCTTCCTCCTCTTGCTGCTGCTGCTCTGCATCCAGCTCGGGGGCGGACAGAAGAAAAAGGAGAA CCTTTTGGCTGAAAAAGTGGAGCAGCTGATGGAATGGAGTTCCAGGCGCTCGATCTTCAGAATGAACGGC GACAAGTTCCGGAAGTTTGTAAAAGCCCCACCTCGGAACTACTCCATGATCGTCATGTTCACTGCTCTGC AGCCTCAGCGGCAGTGTTCTGTGTGCAGGCAGGCTAACGAAGAATATCAAATCCTGGCTAATTCCTGGCG TTATTCATCTGCTTTTTGCAACAAACTGTTTTTTGGAATGGTGGACTATGATGAAGGGACAGATGTTTTT CAACAGCTCAACATGAACTCCGCTCCCACATTCATGCATTTTCCTTCAAAAGGCAGACCCAAGAGAGCTG ATACTTTTGACCTTCAACGAATTGGATTTGCAGCTGAGCAGCTAGCAAAATGGATTGCCGACAGGACGGA TGTTCATATTCGAGTTTTCCGGCCACCCAACTACTCAGGCACCATTGCTTTGGCCCTGTTAGTGTCACTG GTTGGTGGCTTGCTTTATCTAAGGAGGAACAACTTGGAGTTTATCTATAACAAGACTGGTTGGGCCATGG TATCTCTGTGTATAGTCTTTGCTATGACGTCTGGCCAGATGTGGAATCATATCCGTGGACCTCCATATGC TCATAAGAACCCACACAATGGACAAGTGAGCTACATTCATGGAAGCAGCCAGGCTCAGTTTGTGGCAGAG TCACACATCATTCTAGTACTGAATGCTGCTATCACCATGGGGATGGTTCTTCTAAATGAAGCAGCAACTT CCAAAGGGGATGTCGGGAAAAGACGCATCATTTGCCTCGTGGGATTGGGCTTGGTGGTCTTCTTCTTCAG TTTTCTCCTTTCAATATTTCGTTCAAAGTACCATGGCTATCCATATAGCTTTTTAATTAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205219 protein sequence
Red=Cloning site Green=Tags(s) MSARAAPSRRRQAGRRLRYLPTGSFPFLLLLLLLCIQLGGGQKKKENLLAEKVEQLMEWSSRRSIFRMNG DKFRKFVKAPPRNYSMIVMFTALQPQRQCSVCRQANEEYQILANSWRYSSAFCNKLFFGMVDYDEGTDVF QQLNMNSAPTFMHFPSKGRPKRADTFDLQRIGFAAEQLAKWIADRTDVHIRVFRPPNYSGTIALALLVSL VGGLLYLRRNNLEFIYNKTGWAMVSLCIVFAMTSGQMWNHIRGPPYAHKNPHNGQVSYIHGSSQAQFVAE SHIILVLNAAITMGMVLLNEAATSKGDVGKRRIICLVGLGLVVFFFSFLLSIFRSKYHGYPYSFLIK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_030254 |
| ORF Size | 1044 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_030254.4 |
| RefSeq Size | 1527 bp |
| RefSeq ORF | 1044 bp |
| Locus ID | 80286 |
| UniProt ID | Q8BTV1 |
| MW | 39.5 kDa |
| Gene Summary | Acts as accessory component of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. Involved in N-glycosylation of STT3B-dependent substrates. Specifically required for the glycosylation of a subset of acceptor sites that are near cysteine residues; in this function seems to act redundantly with MAGT1. In its oxidized form proposed to form transient mixed disulfides with a glycoprotein substrate to facilitate access of STT3B to the unmodified acceptor site. Has also oxidoreductase-independent functions in the STT3B-containing OST complex possibly involving substrate recognition.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC207781 | Tusc3 (untagged) - Mouse tumor suppressor candidate 3 (Tusc3), (10ug) |
CNY 3,990.00 |
|
| MG205219 | Tusc3 (tGFP-tagged) - Mouse tumor suppressor candidate 3 (Tusc3) |
CNY 3,710.00 |
|
| MR205219L3 | Lenti ORF clone of Tusc3 (Myc-DDK-tagged) - Mouse tumor suppressor candidate 3 (Tusc3) |
CNY 5,230.00 |
|
| MR205219L4 | Lenti ORF clone of Tusc3 (mGFP-tagged) - Mouse tumor suppressor candidate 3 (Tusc3) |
CNY 5,230.00 |
