Dtx3 (NM_030714) Mouse Tagged ORF Clone

CAT#: MR205239

  • TrueORF®

Dtx3 (Myc-DDK-tagged) - Mouse deltex 3 homolog (Drosophila) (Dtx3)

ORF Plasmid: DDK tGFP



  "NM_030714" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,656.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Goat Anti-DTX3 Antibody
    • 100 ug

CNY 5,371.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms Deltex3; mg310
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR205239 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGTTCGTCCTGTCCAGAATGGCAGCCTGTGGAGGCTCCTGCAAGAACAAAGTGACTGTCTCCAAGC
CTGTGTGGGACTTCCTGAGCAAGGAGACCCCAGCCCGGCTGGCCCGGCTTCGGGAGGAGCATCGGGTGTC
CATCCTCATAGATGGCGAGACTTCTGACATCTACGTGCTCCAGCTGTCCCCACAGGGTCCTCCCCCAGCC
CCTCCCAATGGGCTCTACCTGGCCAGGAAGGCTCTCAAGGGGCTACTGAAAGAGGCAGAGAAAGAGCTAA
AGAAAGCCCAGAGGCAGGGGGAGCTCATGGGCTGCCTGGCTTTGGGGGGTGGAGGGGAACACCCCGAGCT
GCACCGCCCAGGCCCTCCTCCCCTCCGAGCAGCCCCACTCCTGCCCCCAGGGGCAAGAGGGCTACCCCCT
CCTCCTCCTCCCCTGCCCCCTCCACTCCCTCCTCGTCTCAGAGAGGACGCTGAAGAGCAGGAAACCACCT
GCCCCATTTGCCTGGGGGAGATACAGAATGCCAAGACGCTGGAGAAGTGCAGGCACTCCTTCTGTGAGGG
CTGCATCACACGGGCCCTGCAGGTGAAAAAGGCCTGCCCCATGTGCGGCCGATTCTACGGGCAGCTTGTG
GGCAACCAGCCCCAGAACGGACGGATGCTGGTCTCCAAGGATGCCACCCTCCTGCTGCCCAGCTACGAGA
AGTATGGCACCATTGTCATCCAATATGTCTTCCCGCCTGGCGTCCAGGGGGCTGAACACCCCAACCCAGG
AGTTCGGTACCCTGGTACCACACGGGTGGCCTACCTCCCGGACTGCCCCGAGGGCAACAAGGTGCTGACC
CTGTTCCGAAAGGCATTTGACCAGCGCCTCACCTTCACCATCGGCACGTCCATGACTACAGGGAGACCCA
ATGTCATCACTTGGAACGACATCCACCACAAGACCAGCTGCACAGGGGGACCCCAGCTGTTTGGGTATCC
GGACCCAACCTACCTGACTAGGGTGCAAGAGGAACTGAGAGCCAAGGGTATCACAGACGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR205239 protein sequence
Red=Cloning site Green=Tags(s)

MSFVLSRMAACGGSCKNKVTVSKPVWDFLSKETPARLARLREEHRVSILIDGETSDIYVLQLSPQGPPPA
PPNGLYLARKALKGLLKEAEKELKKAQRQGELMGCLALGGGGEHPELHRPGPPPLRAAPLLPPGARGLPP
PPPPLPPPLPPRLREDAEEQETTCPICLGEIQNAKTLEKCRHSFCEGCITRALQVKKACPMCGRFYGQLV
GNQPQNGRMLVSKDATLLLPSYEKYGTIVIQYVFPPGVQGAEHPNPGVRYPGTTRVAYLPDCPEGNKVLT
LFRKAFDQRLTFTIGTSMTTGRPNVITWNDIHHKTSCTGGPQLFGYPDPTYLTRVQEELRAKGITDD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_030714
ORF Size 1044 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_030714.2, NP_109639.1
RefSeq Size 2030 bp
RefSeq ORF 1044 bp
Locus ID 80904
UniProt ID Q80V91
MW 38 kDa
Gene Summary Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context. Functions as a ubiquitin ligase protein in vitro, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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