Gale (NM_178389) Mouse Tagged ORF Clone
CAT#: MR205246
- TrueORF®
Gale (Myc-DDK-tagged) - Mouse galactose-4-epimerase, UDP (Gale)
ORF Plasmid: tGFP
"NM_178389" in other vectors (4)
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CNY 3,656.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2310002A12Rik; AI323962 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR205246 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAAGGTGCTGGTCACAGGTGGGGCTGGCTACATCGGCAGCCACACGGTATTGGAGCTGCTGGAGG CAGGCTACTCACCTGTGGTCATTGACAACTTCCATAACGCCATTCGTGGAGAAGACTCCATGCCTGAGAG CCTGCGCCGGGTCCAGGAGTTGACAGGCCGCTCTGTGGAGTTTGAGGAGATGGACATCTTGGACCAGGCA GCCCTACAGCACCTCTTTAAGAAGCACAGCTTTAAGGCCGTCATCCACTTTGCTGGCCTCAAGGCTGTGG GCGAGTCAGTGCAGAAGCCTCTGGACTACTATAGAGTTAACTTAACAGGGACCATCCAGCTTCTAGAGAT CATGAGGGCCCACGGAGTGAAGAACCTGGTGTTCAGCAGCTCAGCCACCGTGTACGGGAACCCCCAGTAC CTGCCTCTGGATGAGGCCCACCCCACGGGGGGCTGTACCAACCCCTACGGCAAGTCCAAGTTCTTCATCG AGGAGATGATCCGGGACCTGTGCCGGGCAGACACGGCCTGGAACGCCGTGCTGCTTCGGTACTTCAATCC CATAGGCGCCCACGCCTCTGGGCGCATCGGTGAAGATCCTCAGGGTATTCCCAACAACCTCATGCCCTAC GTCTCCCAGGTGGCAATCGGGCGACGAGAGGCCCTGAATGTCTTTGGTGATGACTATGCTACAGAGGACG GGACAGGTGTGAGGGATTACATTCACGTGGTGGACCTGGCGAAGGGCCATATAGCAGCCTTGAAGAAGCT GAAGGAGCAATGTGGTTGCCGGACCTACAACCTGGGCACGGGCACAGGCTACTCTGTCCTGCAGATGGTC CAAGCCATGGAGAAAGCCTCAGGGAAGAAGATCCCGTACAAGGTGGTGGCACGGCGGGAAGGTGATGTGG CGGCCTGTTATGCCAACCCCAGCCTGGCCCATGAGGAGCTGGGCTGGACAGCAGCCCTGGGGCTGGACAG GATGTGTGAAGATCTGTGGCGCTGGCAGAAGCAGAACCCTTCGGGCTTTGGGGCGCAGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205246 protein sequence
Red=Cloning site Green=Tags(s) MEKVLVTGGAGYIGSHTVLELLEAGYSPVVIDNFHNAIRGEDSMPESLRRVQELTGRSVEFEEMDILDQA ALQHLFKKHSFKAVIHFAGLKAVGESVQKPLDYYRVNLTGTIQLLEIMRAHGVKNLVFSSSATVYGNPQY LPLDEAHPTGGCTNPYGKSKFFIEEMIRDLCRADTAWNAVLLRYFNPIGAHASGRIGEDPQGIPNNLMPY VSQVAIGRREALNVFGDDYATEDGTGVRDYIHVVDLAKGHIAALKKLKEQCGCRTYNLGTGTGYSVLQMV QAMEKASGKKIPYKVVARREGDVAACYANPSLAHEELGWTAALGLDRMCEDLWRWQKQNPSGFGAQA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_178389 |
ORF Size | 1044 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_178389.3, NP_848476.1 |
RefSeq Size | 1537 bp |
RefSeq ORF | 1044 bp |
Locus ID | 74246 |
UniProt ID | Q8R059 |
MW | 38.2 kDa |
Gene Summary | Catalyzes two distinct but analogous reactions: the reversible epimerization of UDP-glucose to UDP-galactose and the reversible epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The reaction with UDP-Gal plays a critical role in the Leloir pathway of galactose catabolism in which galactose is converted to the glycolytic intermediate glucose 6-phosphate. It contributes to the catabolism of dietary galactose and enables the endogenous biosynthesis of both UDP-Gal and UDP-GalNAc when exogenous sources are limited. Both UDP-sugar interconversions are important in the synthesis of glycoproteins and glycolipids.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC205432 | Gale (untagged) - Mouse galactose-4-epimerase, UDP (Gale), (10ug) |
CNY 3,656.00 |
|
MG205246 | Gale (tGFP-tagged) - Mouse galactose-4-epimerase, UDP (Gale) |
CNY 3,710.00 |
|
MR205246L3 | Lenti ORF clone of Gale (Myc-DDK-tagged) - Mouse galactose-4-epimerase, UDP (Gale) |
CNY 5,230.00 |
|
MR205246L4 | Lenti ORF clone of Gale (mGFP-tagged) - Mouse galactose-4-epimerase, UDP (Gale) |
CNY 5,230.00 |