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Asna1 (NM_019652) Mouse Tagged ORF Clone
CAT＃: MR205259
- TrueORF®
Asna1 (Myc-DDK-tagged) - Mouse arsA arsenite transporter, ATP-binding, homolog 1 (bacterial) (Asna1)
ORF Plasmid: tGFP
"NM_019652" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1810048H22Rik; ArsA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR205259 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGGGGTGGCCGGGTGGGGGGTTGAAGCAGAAGAGTTCGAGGATGCACCTGATGTGGAGCCGC TGGAACCCACGCTTAGCAATATCATCGAGCAGCGTAGCCTTAAGTGGATCTTTGTCGGGGGCAAGGGTGG CGTTGGTAAGACCACCTGCAGCTGCAGCCTGGCAGTCCAGCTGTCGAAGGGACGTGAGAGTGTTCTGATC ATTTCCACAGACCCAGCTCACAACATCTCAGATGCTTTTGACCAGAAGTTCTCCAAGGTGCCTACCAAGG TCAAAGGCTATGACAACCTCTTTGCTATGGAGATAGACCCCAGCCTGGGCGTTGCAGAGCTCCCCGACGA GTTCTTCGAGGAAGACAACATGCTGAGCATGGGCAAGAAGATGATGCAGGAGGCCATGAGCGCCTTCCCT GGCATCGATGAAGCCATGAGTTATGCTGAGGTCATGAGGCTGGTGAAAGGCATGAACTTCTCAGTGGTAG TGTTCGACACAGCACCCACCGGCCATACACTCAGGCTCCTGAACTTCCCGACCATCGTGGAGAGGGGCCT GGGCCGCCTGATGCAGATCAAGAACCAGATCAGCCCCTTCATCTCACAGATGTGCAACATGCTGGGCCTG GGGGACATGAATGCTGACCAACTGGCCTCCAAGCTAGAAGAGACGTTGCCTGTCATCCGATCTGTTAGCG AACAGTTCAAGGACCCTGAACAGACAACGTTCATCTGTGTGTGCATCGCCGAATTCTTGTCCTTGTACGA GACGGAGCGGCTGATCCAGGAGCTGGCTAAGTGCAAGATCGACACCCACAACATCATCGTCAACCAGCTT GTCTTCCCTGACCCTGAGAAACCCTGCAAGATGTGTGAGGCCCGACACAAGATCCAGGCCAAGTACCTGG ACCAGATGGAAGACCTATATGAAGACTTTCACATTGTAAAGCTGCCACTGTTACCTCACGAGGTTCGGGG AGCCGACAAAGTCAACACCTTCTCTGCCCTCCTCCTGGAGCCCTACAAGCCCCCCAGCACCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205259 protein sequence
Red=Cloning site Green=Tags(s) MAAGVAGWGVEAEEFEDAPDVEPLEPTLSNIIEQRSLKWIFVGGKGGVGKTTCSCSLAVQLSKGRESVLI ISTDPAHNISDAFDQKFSKVPTKVKGYDNLFAMEIDPSLGVAELPDEFFEEDNMLSMGKKMMQEAMSAFP GIDEAMSYAEVMRLVKGMNFSVVVFDTAPTGHTLRLLNFPTIVERGLGRLMQIKNQISPFISQMCNMLGL GDMNADQLASKLEETLPVIRSVSEQFKDPEQTTFICVCIAEFLSLYETERLIQELAKCKIDTHNIIVNQL VFPDPEKPCKMCEARHKIQAKYLDQMEDLYEDFHIVKLPLLPHEVRGADKVNTFSALLLEPYKPPSTQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019652 |
| ORF Size | 1047 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_019652.1, NP_062626.1 |
| RefSeq Size | 1280 bp |
| RefSeq ORF | 1047 bp |
| Locus ID | 56495 |
| UniProt ID | O54984 |
| MW | 38.8 kDa |
| Gene Summary | ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202534 | Asna1 (untagged) - Mouse arsA arsenite transporter, ATP-binding, homolog 1 (bacterial) (Asna1), (10ug) |
CNY 3,656.00 |
|
| MG205259 | Asna1 (tGFP-tagged) - Mouse arsA (bacterial) arsenite transporter, ATP-binding, homolog 1 (Asna1) |
CNY 3,140.00 |
|
| MR205259L3 | Lenti ORF clone of Asna1 (Myc-DDK-tagged) - Mouse arsA arsenite transporter, ATP-binding, homolog 1 (bacterial) (Asna1) |
CNY 4,750.00 |
|
| MR205259L4 | Lenti ORF clone of Asna1 (mGFP-tagged) - Mouse arsA arsenite transporter, ATP-binding, homolog 1 (bacterial) (Asna1) |
CNY 4,750.00 |
