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Kmt5a (NM_030241) Mouse Tagged Lenti ORF Clone
CAT＃: MR205297L4
- LentiORF®
Lenti ORF clone of Setd8 (mGFP-tagged) - Mouse SET domain containing (lysine methyltransferase) 8 (Setd8)
"NM_030241" in other vectors (4)
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CNY 5,230.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | mGFP |
| Synonyms | 2410195B05Rik; AA617402; AW536475; PR-SET7; PR/SET07; Setd8 |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR205297).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_030241 |
| ORF Size | 1050 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_030241.3, NP_084517.2 |
| RefSeq Size | 2742 bp |
| RefSeq ORF | 1053 bp |
| Locus ID | 67956 |
| UniProt ID | Q2YDW7 |
| Gene Summary | Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. Plays a negative role in TGF-beta response regulation and a positive role in cell migration (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC210666 | Setd8 (untagged) - Mouse SET domain containing (lysine methyltransferase) 8 (Setd8), (10ug) |
CNY 3,656.00 |
|
| MG205297 | Setd8 (tGFP-tagged) - Mouse SET domain containing (lysine methyltransferase) 8 (Setd8) |
CNY 3,710.00 |
|
| MR205297 | Setd8 (Myc-DDK-tagged) - Mouse SET domain containing (lysine methyltransferase) 8 (Setd8) |
CNY 3,330.00 |
|
| MR205297L3 | Lenti ORF clone of Setd8 (Myc-DDK-tagged) - Mouse SET domain containing (lysine methyltransferase) 8 (Setd8) |
CNY 5,230.00 |
