Atp6v0d1 (NM_013477) Mouse Tagged ORF Clone
CAT#: MR205337
- TrueORF®
Atp6v0d1 (Myc-DDK-tagged) - Mouse ATPase, H+ transporting, lysosomal V0 subunit D1 (Atp6v0d1)
ORF Plasmid: tGFP
"NM_013477" in other vectors (4)
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CNY 3,656.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Ac39; AI267038; Atp6d; P39; VATX; Vma6 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR205337 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGTTCTTCCCGGAGCTCTATTTCAACGTGGACAATGGCTACTTGGAGGGATTAGTGCGCGGCCTGA AGGCCGGGGTGCTCAGCCAGGCGGACTACCTCAACCTGGTGCAGTGCGAGACGCTCGAGGACTTGAAGCT GTACCTGCAGAGTACAGATTATGGCAACTTCCTGGCCAATGAAGCGTCACCTCTGACGGTGTCAGTCATC GATGACAAGCTCAAGGAGAAGATGGTAGTAGAGTTCCGCCACATGAGAAACCATGCTTATGAGCCGCTCG CCAGCTTCCTGGACTTCATAACTTATAGCTACATGATTGACAACGTGATCCTGCTAATCACAGGCACACT GCACCAGCGTTCAATAGCTGAACCTGTGCCCAAGTGCCATCCGCTAGGCAGCTTTGAGCAGATGGAGGCT GTGAACATCGCACAGACACCTGCAGAGCTCTACAATGCCATTCTGGTGGACACACCCCTGGCGGCTTTTT TCCAGGACTGCATCTCAGAGCAGGACCTTGATGAGATGAACATCGAGATAATCCGAAATACGCTTTACAA GGCCTACTTGGAGTCCTTCTACAAGTTCTGTACTCTGTTGGGTGGGACCACAGCTGATGCCATGTGTCCT ATCCTAGAGTTTGAAGCAGACCGCCGCGCTTTCATCATCACCATCAACTCTTTCGGCACAGAGCTGTCCA AGGAGGACCGTGCCAAGCTCTTCCCGCACTGTGGCCGGCTCTACCCTGAGGGCTTGGCTCAGCTGGCGCG GGCTGATGACTATGAACAGGTCAAGAATGTAGCTGATTACTACCCGGAATACAAACTGCTTTTTGAGGGT GCAGGTAGCAATCCTGGAGACAAGACCCTGGAGGACCGGTTTTTTGAACATGAGGTGAAGCTAAACAAGC TGGCCTTCCTGAACCAGTTCCATTTTGGTGTCTTTTATGCCTTTGTGAAGCTTAAGGAACAGGAATGTCG CAACATCGTATGGATTGCTGAGTGCATTGCCCAGCGCCATCGCGCCAAGATCGACAACTACATCCCCATC TTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205337 protein sequence
Red=Cloning site Green=Tags(s) MSFFPELYFNVDNGYLEGLVRGLKAGVLSQADYLNLVQCETLEDLKLYLQSTDYGNFLANEASPLTVSVI DDKLKEKMVVEFRHMRNHAYEPLASFLDFITYSYMIDNVILLITGTLHQRSIAEPVPKCHPLGSFEQMEA VNIAQTPAELYNAILVDTPLAAFFQDCISEQDLDEMNIEIIRNTLYKAYLESFYKFCTLLGGTTADAMCP ILEFEADRRAFIITINSFGTELSKEDRAKLFPHCGRLYPEGLAQLARADDYEQVKNVADYYPEYKLLFEG AGSNPGDKTLEDRFFEHEVKLNKLAFLNQFHFGVFYAFVKLKEQECRNIVWIAECIAQRHRAKIDNYIPI F TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_013477 |
ORF Size | 1056 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_013477.1, NM_013477.2, NM_013477.3, NP_038505.2 |
RefSeq Size | 1617 bp |
RefSeq ORF | 1056 bp |
Locus ID | 11972 |
UniProt ID | P51863 |
MW | 40.3 kDa |
Gene Summary | Subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. May play a role in coupling of proton transport and ATP hydrolysis. May play a role in cilium biogenesis through regulation of the transport and the localization of proteins to the cilium (By similarity). In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200877 | Atp6v0d1 (untagged) - Mouse ATPase, H+ transporting, lysosomal V0 subunit D1 (Atp6v0d1), (10ug) |
CNY 3,656.00 |
|
MG205337 | Atp6v0d1 (tGFP-tagged) - Mouse ATPase, H+ transporting, lysosomal V0 subunit D1 (Atp6v0d1) |
CNY 3,140.00 |
|
MR205337L3 | Lenti ORF clone of Atp6v0d1 (Myc-DDK-tagged) - Mouse ATPase, H+ transporting, lysosomal V0 subunit D1 (Atp6v0d1) |
CNY 4,750.00 |
|
MR205337L4 | Lenti ORF clone of Atp6v0d1 (mGFP-tagged) - Mouse ATPase, H+ transporting, lysosomal V0 subunit D1 (Atp6v0d1) |
CNY 4,750.00 |
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