United States
Germany
Japan
United Kingdom
China
Car12 (NM_178396) Mouse Tagged ORF Clone
CAT＃: MR205383
- TrueORF®
Car12 (Myc-DDK-tagged) - Mouse carbonic anyhydrase 12 (Car12)
ORF Plasmid: tGFP
"NM_178396" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2310047E01Rik; AI314958; CA-XII; Ca12 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR205383 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCACCGCAGCCTGCGCGCGACTGTCGTCCTCCTGTTGGTGATCCTTAAGAAGCAGCCTTCCAGCT CAGCCCCACTCAACGGATCCAAGTGGACCTATGTTGGTCCTGCTGGGGAAAAGAACTGGTCCAAGAAGTA CCCATCGTGTGGAGGCCTCCTGCAGTCCCCGATAGACCTACATAGCGACATCCTTCAGTATGATGCTAGC CTTGCACCCCTCCAGTTCCAAGGTTACAACGTGTCTGTTGAAAAGCTGTTGAACCTGACCAATGATGGCC ATTCAGTGAGACTGAATCTGAACTCAGACATGTACATCCAGGGTCTCCAGCCTCACCACTACAGAGCAGA GCAGCTGCACTTGCACTGGGGGAACCGCAATGACCCCCATGGCTCTGAACACACCGTGAGTGGGAAGCAC TTTGCTGCCGAGCTGCACATTGTCCATTATAACTCAGACCTGTACCCTGACTTCAGCACCGCCAGTGACA AGTCCGAAGGCCTCGCTGTCCTTGCTGTTCTTATTGAGATAGGCTCCGCCAATCCGTCATATGACAAGAT CTTCAGCCATCTTCAACATGTTAAGTATAAAGGCCAACAGGTGCTCATCCCAGGCTTCAACATTGAAGAA CTTCTGCCCGAGAGCCCTGGCGAATATTACCGCTACGAAGGGTCCCTGACTACACCTCCTTGCTACCCTA CCGTGCTCTGGACAGTTTTCCGAAACCCTGTGCAGATTTCCCAGGAGCAGCTGCTGGCTTTGGAGACAGC TCTGTACTTCACACACATGGATGATCCTACCCCCAGAGAAATGATCAACAACTTCCGGCAGGTCCAGAAG TTCGATGAGAGGCTGGTGTATATCTCCTTCCGACAAGGACTGCTTACCGACACAGGACTGAGTTTGGGTA TCATCCTTTCAGTGGCCCTGGCTGGCGTTCTTGGCATCTCCATTGTTCTGGCAGTGTCTATTTGGCTCTT CAAAAGGAAGAAGAGCAAAAAAGGTGACAACAAGGGAGTCATTTATAAACCAGCCATCAAGAAGGAGGCT GAGGTCCACGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205383 protein sequence
Red=Cloning site Green=Tags(s) MPHRSLRATVVLLLVILKKQPSSSAPLNGSKWTYVGPAGEKNWSKKYPSCGGLLQSPIDLHSDILQYDAS LAPLQFQGYNVSVEKLLNLTNDGHSVRLNLNSDMYIQGLQPHHYRAEQLHLHWGNRNDPHGSEHTVSGKH FAAELHIVHYNSDLYPDFSTASDKSEGLAVLAVLIEIGSANPSYDKIFSHLQHVKYKGQQVLIPGFNIEE LLPESPGEYYRYEGSLTTPPCYPTVLWTVFRNPVQISQEQLLALETALYFTHMDDPTPREMINNFRQVQK FDERLVYISFRQGLLTDTGLSLGIILSVALAGVLGISIVLAVSIWLFKRKKSKKGDNKGVIYKPAIKKEA EVHA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_178396 |
| ORF Size | 1065 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_178396.3, NP_848483.2 |
| RefSeq Size | 3716 bp |
| RefSeq ORF | 1065 bp |
| Locus ID | 76459 |
| UniProt ID | Q8CI85 |
| MW | 39.7 kDa |
| Gene Summary | This gene encodes a membrane-bound member of the alpha carbonic anhydrase family of enzymes that catalyze the reversible hydration of carbon dioxide to bicarbonate. These proteins participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC211740 | Car12 (untagged) - Mouse carbonic anyhydrase 12 (Car12), (10ug) |
CNY 5,488.00 |
|
| MG205383 | Car12 (tGFP-tagged) - Mouse carbonic anyhydrase 12 (Car12) |
CNY 4,370.00 |
|
| MR205383L3 | Lenti ORF clone of Car12 (Myc-DDK-tagged) - Mouse carbonic anyhydrase 12 (Car12) |
CNY 5,890.00 |
|
| MR205383L4 | Lenti ORF clone of Car12 (mGFP-tagged) - Mouse carbonic anyhydrase 12 (Car12) |
CNY 5,890.00 |
