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St8sia4 (NM_009183) Mouse Tagged ORF Clone
CAT＃: MR205502
- TrueORF®
St8sia4 (Myc-DDK-tagged) - Mouse ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4 (St8sia4), transcript variant 1
ORF Plasmid: tGFP
"NM_009183" in other vectors (4)
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CNY 3,656.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PST; PST-1; SIAT8-D; Siat8d; ST8SiaIV |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR205502 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCTCAATTAGAAAACGGTGGACCATCTGCACTATAAGTCTACTCCTGATCTTTTATAAGACAAAAG AAATAGCCAGAACTGAGGAGCACCAAGAGACGCAACTCATCGGAGATGGTGAACTGTGTTTGAGCAGATC ACTTGTCAACAGCTCTGATAAAATCATCCGGAAGGCTGGCTCCACCATCTTCCAACATTCTGTACAAGGC TGGAAAATCAATTCTTCTTTAGTCCTGGAGATAAGGAAGAACATTCTTCGTTTCTTAGATGCGGAACGTG ACGTATCTGTGGTCAAGAGCAGTTTTAAGCCTGGTGATGTCATACACTATGTGCTGGACAGACGCCGGAC ACTAAATATTTCTCACAATCTGCATAGCCTCCTGCCTGAAGTTTCACCAATGAAAAACCGCAGGTTTAAG ACCTGTGCAGTTGTTGGAAATTCCGGCATTCTGCTAGACAGTGGATGTGGAAAGGAGATTGACAGCCACA ACTTTGTAATAAGGTGCAATCTAGCTCCTGTGGTGGAGTTCGCTGCTGATGTGGGGACTAAATCAGATTT TATTACCATGAATCCATCAGTTGTGCAAAGAGCATTTGGAGGCTTTCGGAATGAGAGTGACAGAGAAAAA TTTGTGCATAGACTCTCCATGCTGAATGACAGCGTCCTTTGGATTCCTGCCTTCATGGTCAAAGGAGGAG AGAAGCACGTGGAGTGGGTTAATGCATTAATCCTTAAGAACAAACTGCAAGTGCGAACTGCCTATCCATC ACTTAGACTTATTCATGCAGTCAGAGGATACTGGCTGACTAACAAAGTTCCTATCAAAAGACCTAGCACA GGTCTCCTCATGTATACACTTGCCACCAGATTCTGTGATGAAATTCACCTTTATGGATTCTGGCCATTTC CCAAGGATCTGAATGGAAAAGCTGTCAAATATCATTACTATGATGACTTAAAATATAGATACTTTTCCAA CGCCAGTCCTCACAGGATGCCATTAGAATTTAAAACATTGAGTGTGCTACACAATAGAGGAGCTCTAAAA CTGACCACAGGAAAGTGCATGAAGCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205502 protein sequence
Red=Cloning site Green=Tags(s) MRSIRKRWTICTISLLLIFYKTKEIARTEEHQETQLIGDGELCLSRSLVNSSDKIIRKAGSTIFQHSVQG WKINSSLVLEIRKNILRFLDAERDVSVVKSSFKPGDVIHYVLDRRRTLNISHNLHSLLPEVSPMKNRRFK TCAVVGNSGILLDSGCGKEIDSHNFVIRCNLAPVVEFAADVGTKSDFITMNPSVVQRAFGGFRNESDREK FVHRLSMLNDSVLWIPAFMVKGGEKHVEWVNALILKNKLQVRTAYPSLRLIHAVRGYWLTNKVPIKRPST GLLMYTLATRFCDEIHLYGFWPFPKDLNGKAVKYHYYDDLKYRYFSNASPHRMPLEFKTLSVLHNRGALK LTTGKCMKQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009183 |
| ORF Size | 1080 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_009183.1, NM_009183.2, NP_033209.1 |
| RefSeq Size | 5437 bp |
| RefSeq ORF | 1080 bp |
| Locus ID | 20452 |
| UniProt ID | Q64692 |
| MW | 41.2 kDa |
| Gene Summary | Catalyzes the polycondensation of alpha-2,8-linked sialic acid required for the synthesis of polysialic acid (PSA), which is present on the embryonic neural cell adhesion molecule (N-CAM), necessary for plasticity of neural cells.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The Prion Protein Controls Polysialylation of Neural Cell Adhesion Molecule 1 during Cellular Morphogenesis
,Mehrabian, M;Brethour, D;Wang, H;Xi, Z;Rogaeva, E;Schmitt-Ulms, G;,
PLoS ONE
,PubMed ID 26288071
[ST8SIA4]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202588 | St8sia4 (untagged) - Mouse ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4 (St8sia4), transcript variant 1, (10ug) |
CNY 3,656.00 |
|
| MG205502 | St8sia4 (tGFP-tagged) - Mouse ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4 (St8sia4) |
CNY 3,710.00 |
|
| MR205502L3 | Lenti ORF clone of St8sia4 (Myc-DDK-tagged) - Mouse ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4 (St8sia4), transcript variant 1 |
CNY 5,230.00 |
|
| MR205502L4 | Lenti ORF clone of St8sia4 (mGFP-tagged) - Mouse ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4 (St8sia4), transcript variant 1 |
CNY 6,056.00 |
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